Please use this identifier to cite or link to this item:
https://observatorio.fm.usp.br/handle/OPI/41730
Title: | Evaluation of the neuromuscular junction in a middle-aged mouse model of congenital myasthenic syndrome |
Authors: | SCHETINO, Luana Pereira Leite; FONSECA, Matheus de Castro; GOMES, Matheus Proenca Simao Magalhaes; VALADAO, Priscila Aparecida Costa; CAMARGO, Wallace Lucio de; RODRIGUES, Hermann Alecsandro; ANDRADE, Jessica Neves; ARANTES-COSTA, Fernanda Magalhaes; NAVES, Ligia Araujo; PRADO, Carla Maximo; PRADO, Vania Ferreira; PRADO, Marco Antonio Maximo; GUATIMOSIM, Cristina |
Citation: | MUSCLE & NERVE, v.60, n.6, p.790-800, 2019 |
Abstract: | Introduction Reduced expression of the vesicular acetylcholine transporter (VAChT) leads to changes in the distribution and shape of synaptic vesicles (SVs) at neuromuscular junctions (NMJs), suggesting vesicular acetylcholine (ACh) as a key component of synaptic structure and function. It is poorly understood how long-term changes in cholinergic transmission contribute to age- and disease-related degeneration in the motor system. Methods In this study we performed confocal imaging, electrophysiology, electron microscopy, and analyses of respiratory mechanics of the diaphragm NMJ components in 12-month-old wild-type (WT) and VAChTKD(HOM) mice. Results Diaphragms of NMJs of the VAChTKD(HOM) mice were similar to those in WT mice in number, colocalization, and fragmentation of pre-/postsynaptic components. However, they had increased spontaneous SV exocytosis, miniature endplate potential frequency, and diminished MEPP amplitude. No impairment in respiratory mechanics at rest was observed, probably due to the large neurotransmission safety factor of the diaphragm. Discussion The present findings help us to understand the consequences of reduced ACh release at the NMJs during aging. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/20 |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
art_SCHETINO_Evaluation_of_the_neuromuscular_junction_in_a_middleaged_2019.PDF Restricted Access | publishedVersion (English) | 4.95 MB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.