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Title: Immunomodulatory effects of thalidomide in an experimental brain death liver donor model
Authors: SANTANA, Alexandre ChagasANDRAUS, WellingtonSILVA, Filipe Miranda OliveiraDELLE, HumbertoPEPINELI, RafaelMORAES, Edvaldo Leal deSCAVONE, CristoforoLIMA, Larissa de SaDEGASPARI, SabrinaBRASIL, SergioSOLLA, Davi Jorge FontouraRUIZ, Liliane MoreiraOLIVEIRA-BRAGA, Karina Andrighetti deNEPOMUCENO, Natalia AparecidaPEGO-FERNANDES, Paulo ManuelTULLIUS, Stefan GuntherFIGUEIREDO, Eberval Gadelha
Citation: SCIENTIFIC REPORTS, v.11, n.1, article ID 19221, 13p, 2021
Abstract: Brain death is characterized by a generalized inflammatory response that results in multiorgan damage. This process is mainly mediated through cytokines, which amplify graft immunogenicity. We investigated the immunological response in a brain death liver donor model and analysed the effects of thalidomide, a drug with powerful immunomodulatory properties. Brain death was induced in male Lewis rats. We studied three groups: Control (sham-operated rats in which trepanation was performed without inserting the balloon catheter), BD (rats subjected to brain death by increasing intracranial pressure) and BD + Thalid (BD rats receiving thalidomide after brain death). After 6 h, serum levels of AST, ALT, LDH, and ALP as well as systemic and hepatic levels of TNF-alpha, IL1-beta, IL-6, and IL-10 were analysed. We also determined the mRNA expression of MHC Class I and Class II, NF-kappa B, and macrophage infiltration. NF-kappa B was also examined by electrophoretic mobility shift assay. Thalidomide treatment significantly reduced serum levels of hepatic enzymes and TNF-alpha, IL-1-beta, and IL-6. These cytokines were evaluated at either the mRNA expression or protein level in liver tissue. In addition, thalidomide administration resulted in a significant reduction in macrophages, MHC Class I and Class II, and NF-kappa B activation. This study reveals that thalidomide significantly inhibited the immunologic response and graft immunogenicity, possibly through suppression of NF-kappa B activation.
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Artigos e Materiais de Revistas Científicas - FM/MCP
Departamento de Cardio-Pneumologia - FM/MCP

Artigos e Materiais de Revistas Científicas - FM/MNE
Departamento de Neurologia - FM/MNE

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/37
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado

Artigos e Materiais de Revistas Científicas - LIM/45
LIM/45 - Laboratório de Fisiopatologia Neurocirúrgica

Artigos e Materiais de Revistas Científicas - LIM/61
LIM/61 - Laboratório de Pesquisa em Cirurgia Torácica

Artigos e Materiais de Revistas Científicas - LIM/62
LIM/62 - Laboratório de Fisiopatologia Cirúrgica

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