Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/4278
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorRONDON, Fernanda Cristina Macedo-
dc.contributor.authorBEVILAQUA, Claudia Maria Leal-
dc.contributor.authorACCIOLY, Marina Parissi-
dc.contributor.authorMORAIS, Selene Maia de-
dc.contributor.authorANDRADE-JUNIOR, Heitor Franco de-
dc.contributor.authorCARVALHO, Camila Aparecida de-
dc.contributor.authorLIMA, Josemar Coelho-
dc.contributor.authorMAGALHAES, Hilton Cesar Rodrigues-
dc.date.accessioned2014-01-28T22:27:10Z-
dc.date.available2014-01-28T22:27:10Z-
dc.date.issued2012-
dc.identifier.citationREVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v.21, n.3, p.185-191, 2012-
dc.identifier.issn0103-846X-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/4278-
dc.description.abstractThe increased incidence of visceral leishmaniasis (VL) in Brazil is due to a lack of effective disease control measures. In addition to that, no effective treatment exists for canine VL in response to synthetic drugs. Thus, the objective of this study was to evaluate the effect of the essential oils of Coriandrum sativum and Lippia sidoides, and oleoresin from Copaifera reticulata, on Leishmania chagasi promastigotes and amastigotes. We also examined the toxicity of these treatments on the murine monocyte cell line RAW 264.7. To determine the IC50 a MTT test (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was performed on promastigotes, and an in situ ELISA assay was conducted on amastigotes. Here, we demonstrate that oleoresin from C. reticulata was effective against both promastigotes (IC50 of 7.88 mu g.mL(-1)) and amastigotes (IC50 of 0.52 mu g.mL(-1)), and neither of the two treatments differed significantly (p > 0.05) from pentamidine (IC50 of 2.149 mu g.mL(-1)) and amphotericin B (IC50 of 9.754 mu g.mL(-1)). Of the three plant oils tested, only oleoresin showed no toxicity toward monocyte, with 78.45% viability after treatment. Inhibition of promastigote and amastigote growth and the lack of cytotoxicity by C. reticulata demonstrate that oleoresin may be a viable option for analyzing the in vivo therapeutic effects of leishmanicidal plants-
dc.description.abstractO aumento na incidência da Leishmaníase Visceral (LV) no Brasil deve-se à ineficácia das medidas de controle da doença. Além disso, não há tratamento efetivo para LV canina com drogas sintéticas. Assim, o objetivo deste trabalho foi avaliar o efeito dos óleos essenciais de Coriandrum sativum e de Lippia sidoides e do óleo-resina de Copaiferareticulata sobre promastigotas e amastigotas de Leishmania chagasi e analisar o grau de toxicidade sobre células monocíticas murinas RAW 264.7. Para determinar a CI50 sobre promastigotas foi usado teste MTT (brometo de 3-[4,5-dimetil-tiazol-2-il]-2,5-difeniltetrazólio) e sobre amastigotas foi realizado imunoensaio in situ pela técnica de ELISA. Os resultados obtidos comprovaram que o óleo-resina de C. reticulata foi o mais eficaz contra as formas promastigotas (CI50 de 7,88 µg.mL-1) e amastigotas (CI50 de 0,52 µg.mL-1) e em nenhum dos dois testes diferiu do controle pentamidina que obteve CI50 de 2,149 µg.mL-1, no teste sobre promastigotas, e anfotericina B que obteve CI50 de 9,754 µg.mL-1, nos testes com amastigotas (p > 0.05). Quanto à citotoxicidade apenas o óleo-resina não apresentou toxicidade com 78,45% de monócitos viáveis. Os resultados obtidos sobre promastigotas e amastigotas e a ausência de citotoxicidade do óleo-resina de C. reticulata evidenciam que este óleo-resina pode ser viável para a análise de seus efeitos terapêuticos em testes in vivo.-
dc.description.sponsorshipCNPq-
dc.description.sponsorshipFUNCAP-
dc.language.isoeng-
dc.publisherBRAZILIAN COLL VETERINARY PARASITOLOGY-
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria-
dc.rightsopenAccess-
dc.subjectLeishmanicidal plants-
dc.subjectoils-
dc.subjectcytotoxicity-
dc.subjectamastigotes-
dc.subjectpromastigotes-
dc.subjectPlantas leishmanicidas-
dc.subjectóleos-
dc.subjectcitotoxicidade-
dc.subjectamastigotas-
dc.subjectpromastigotas-
dc.subjectLeishmania chagasi-
dc.subject.otheressential oil-
dc.subject.otherlarvicidal activity-
dc.subject.otherchemical-composition-
dc.subject.otherantifungal activity-
dc.subject.othermedicinal-plants-
dc.subject.otheramphotericin-b-
dc.subject.otherdrug targets-
dc.subject.otherl.-
dc.subject.othercham.-
dc.subject.othermode-
dc.titleIn vitro efficacy of Coriandrum sativum, Lippia sidoides and Copaifera reticulata against Leishmania chagasi-
dc.title.alternativeEficácia in vitro de Coriandrum sativum, Lippia sidoides e Copaifera reticulata sobre Leishmania chagasi-
dc.typearticle-
dc.rights.holderCopyright BRAZILIAN COLL VETERINARY PARASITOLOGY-
dc.identifier.doi10.1590/S1984-29612012000300002-
dc.identifier.pmid23070424-
dc.subject.wosParasitology-
dc.subject.wosVeterinary Sciences-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-
hcfmusp.author.externalRONDON, Fernanda Cristina Macedo:Univ Estadual Ceara UECE, Lab Doencas Parasitarias, BR-60714903 Fortaleza, Ceara, Brazil-
hcfmusp.author.externalBEVILAQUA, Claudia Maria Leal:Univ Estadual Ceara UECE, Lab Doencas Parasitarias, BR-60714903 Fortaleza, Ceara, Brazil-
hcfmusp.author.externalACCIOLY, Marina Parissi:Univ Estadual Ceara UECE, Lab Doencas Parasitarias, BR-60714903 Fortaleza, Ceara, Brazil-
hcfmusp.author.externalMORAIS, Selene Maia de:Univ Estadual Ceara UECE, Lab Quim Prod Nat, BR-60714903 Fortaleza, Ceara, Brazil-
hcfmusp.author.externalCARVALHO, Camila Aparecida de:Univ Sao Paulo, Lab Protozool, Sao Paulo, Brazil-
hcfmusp.author.externalLIMA, Josemar Coelho:Embrapa Agroind Trop, Lab Anal Alimentos, Fortaleza, Ceara, Brazil-
hcfmusp.author.externalMAGALHAES, Hilton Cesar Rodrigues:Embrapa Agroind Trop, Lab Anal Alimentos, Fortaleza, Ceara, Brazil-
hcfmusp.description.beginpage185-
hcfmusp.description.endpage191-
hcfmusp.description.issue3-
hcfmusp.description.volume21-
hcfmusp.origemWOS-
hcfmusp.origem.id2-s2.0-84867457279-
hcfmusp.origem.idWOS:000318255300002-
hcfmusp.origem.idSCIELO:S1984-29612012000300002-
hcfmusp.publisher.citySAO PAULO-
hcfmusp.publisher.countryBRAZIL-
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dc.description.indexMEDLINE-
dc.identifier.eissn1984-2961-
hcfmusp.remissive.sponsorshipCNPq-
hcfmusp.lim.ref2012-
hcfmusp.citation.scopus27-
hcfmusp.scopus.lastupdate2022-05-20-
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LIM/49 - Laboratório de Protozoologia


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