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Title: NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus
Authors: PEREIRA, Samuel SantosANDREATA-SANTOS, RobertPEREIRA, Lennon RamosSOARES, Camila PereiraFELIX, Alvina ClaraANDRADE, Patricia de Mello Jungmann Cardoso deDURIGON, Edison LuisROMANO, Camila MaltaFERREIRA, Luis Carlos de Souza
Abstract: Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (Delta NS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regions for the serological discrimination of both DENV and ZIKV, such as Brazil and other tropical countries. Methods: The Delta NS1 proteins were obtained as recombinant antigens and were evaluated as solid-phase-bound antigens in the ELISA test to detect anti-NS1 IgG antibodies. The performance of the Delta NS1-based DENV IgG ELISA was assessed with both mouse and human serum samples previously exposed to DENV or ZIKV. Results: The Delta NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive serum samples. The sensitivity and specificity of the assay determined using samples previously characterized by real-time PCR (qRT-PCR) or plaque reduction neutralization assay (PRNT) were 82% and 93%, respectively. Conclusion: The Delta NS1-based DENV IgG ELISA conferred enhanced diagnostic specificity for anti-DENV serological tests and may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission. (C) 2021 The Authors.
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Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar

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