https://observatorio.fm.usp.br/handle/OPI/44458
Título: | SARS-CoV-2 recombinant proteins stimulate distinct cellular and humoral immune response profiles in samples from COVID-19 convalescent patients |
Autor(es): | SILVA, Lais Teodoro da; ORTEGA, Marina Mazzilli; TIYO, Bruna Tiaki; VIANA, Isabelle Freire Tabosa; LIMA, Tayna Evily de; TOZETTO-MENDOZA, Tania Regina; OLIVEIRA, Luanda Mara da Silva; TEIXEIRA, Franciane Mouradian Emidio; LINS, Roberto Dias; ALMEIDA, Alexandre de; MENDES-CORREA, Maria Cassia; DUARTE, Alberto Jose da Silva; OSHIRO, Telma Miyuki |
Parte de: | CLINICS, v.76, article ID e3548, 11p, 2021 |
Resumo: | OBJECTIVES: In this preliminary study we investigated cellular and humoral immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood samples from 14 recovered coronavirus disease 2019 (COVID-19) patients and compared them to those in samples from 12 uninfected/unvaccinated volunteers. METHODS: Cellular immunity was assessed by intracellular detection of IFN-g in CD3+ T lymphocytes after stimulation with SARS-CoV-2 spike (S1), nucleocapsid (NC), or receptor-binding domain (RBD) recombinant proteins or overlapping peptide pools covering the sequence of SARS-CoV-2 spike, membrane and nucleocapsid regions. The humoral response was examined by ELISAs and/or chemiluminescence assays for the presence of serum IgG antibodies directed to SARS-CoV-2 proteins. RESULTS: We observed differences between humoral and cellular immune profiles in response to stimulation with the same proteins. Assays of IgG antibodies directed to SARS-CoV-2 NC, RBD and S1/S2 recombinant proteins were able to differentiate convalescent from uninfected/unvaccinated groups. Cellular immune responses to SARS-CoV-2 protein stimuli did not exhibit a specific response, as T cells from both individuals with no history of contact with SARS-CoV-2 and from recovered donors were able to produce IFN-g. CONCLUSIONS: Determination of the cellular immune response to stimulation with a pool of SARS-CoV-2 peptides but not with SARS-CoV-2 proteins is able to distinguish convalescent individuals from unexposed individuals. Regarding the humoral immune response, the screening for serum IgG antibodies directed to SARSCoV-2 proteins has been shown to be specific for the response of recovered individuals. |
Aparece nas coleções: | Artigos e Materiais de Revistas Científicas - COVID-19 Artigos e Materiais de Revistas Científicas - FM/MIP Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/05 Artigos e Materiais de Revistas Científicas - LIM/52 Artigos e Materiais de Revistas Científicas - LIM/56 Artigos e Materiais de Revistas Científicas - ODS/03 Artigos e Materiais de Revistas Científicas - ODS/10 |
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art_SILVA_SARSCoV2_recombinant_proteins_stimulate_distinct_cellular_and_humoral_2021.PDF | publishedVersion (English) | 1.1 MB | Adobe PDF | Visualizar/Abrir |
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