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Title: Indoleamine 2,3-Dioxygenase-1 Expression is Changed During Bladder Cancer Cell Invasion
Authors: SANTOS, Hellen Joyce Sousa PereiraMATHEUS, Luiz Henrique GomesSILVA, AlineDALMAZZO, Stephanie VaninSANTOS, Andressa AssuncaoSANTOS, Leticia Rafaela Alves RubensSOUZA, Diego MotaREIS, Sabrina ThalitaNASCIMENTO, Ivan PereiraDELLE, Humberto
Citation: INTERNATIONAL JOURNAL OF TRYPTOPHAN RESEARCH, v.15, article ID 11786469211065600, 6p, 2022
Abstract: The severity of the bladder carcinoma (BC) is directly linked to cell invasion and metastasis. Indoleamine 2,3-dioxygenase-1 (IDO-1) is an INF-gamma-induced immunomodulating enzyme that has been linked to the cancer cell invasiveness. Because IDO1 is variable among the tumors, we analyzed its expression in the BC invasion using BC mice models and cell culture. MB49 cells were orthotopically or ectopically inoculated in C57Bl6 mice to evaluate IDO1 by immunohistochemistry. For in vitro experiments, expression of IDO1 and INF-gamma was evaluated in grade-1 (RT4) and in grade-3 (T24) BC cell lines. Invading and non-invading T24 cells were separated using the Matrigel/Transwell system, of which total RNA was extracted immediately or after 2 weeks of subculture. Finally, IDO1 was silenced in T24 cells to verify its role on cell invasiveness. In both animal models, IDO1 was differentially expressed between non-invading and invading cells. In cell culture, T24 cells expressed more IDO1 than RT4 cells, independently of the INF-gamma expression. IDO1 was differentially expressed between non-invading and invading T24 cells, a difference that was lost by long-time subculture. IDO1 silencing resulted in diminished cell invasiveness. In conclusion, IDO1 expression is changed during bladder carcinoma invasion, playing an important role in this process.
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Artigos e Materiais de Revistas Científicas - FM/Outros
Outros departamentos - FM/Outros

Artigos e Materiais de Revistas Científicas - LIM/55
LIM/55 - Laboratório de Urologia

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