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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorARISMENDI, Maria Izabel-
dc.contributor.authorKALLAS, Esper Georges-
dc.contributor.authorSANTOS, Bianca Almeida Natali dos-
dc.contributor.authorCARNEIRO-SAMPAIO, Magda Maria Sales-
dc.contributor.authorKAYSER, Cristiane-
dc.identifier.citationCLINICS, v.67, n.5, p.425-429, 2012-
dc.description.abstractOBJECTIVES: The purpose of this study was to investigate the association between T cell receptor excision circle levels in peripheral blood mononuclear cells and regulatory T cells that co-express CD25 and Foxp3 in healthy children and adolescents of different ages. MATERIALS AND METHODS: The quantification of signal-joint T-cell receptor excision circle levels in the genomic DNA of peripheral blood mononuclear cells was performed using real-time quantitative PCR. The analysis of CD4, CD8, CD25, and Foxp3 expression was performed using flow cytometry. RESULTS: Ninety-five healthy controls (46 females and 49 males) ranging in age from 1 to 18 years were analyzed. The mean T-cell receptor excision circle count in all individuals was 89.095 +/- 36.790 T-cell receptor excision circles per microgram of DNA. There was an inverse correlation between T-cell receptor excision circles counts and age (r = -0.846; p < 0.001) as well as between the proportion of CD4(+)CD25(+)Foxp3(+) T cells and age (r = -0.467; p = 0.04). In addition, we observed a positive correlation between the amount of CD4(+)CD25(+)Foxp3(+) T cells and the amount of T-cell receptor excision circles per microgram of DNA in individuals of all ages (r = -0.529; p = 0.02). CONCLUSIONS: In this study, we observed a decrease in the thymic function with age based on the fact that the level of T-cell receptor excision circles in the peripheral blood positively correlated with the proportion of regulatory T cells in healthy children and adolescents. These findings indicate that although T-cell receptor excision circles and regulatory T cells levels decrease with age, homeostasis of the immune system and relative regulatory T cells population levels are maintained in the peripheral blood.-
dc.description.sponsorshipState of Sao Paulo Research Foundation (FAPESP) [2008/58238-4]-
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel (CAPES)-
dc.subjectT lymphocytes-
dc.subjectT-cell Receptor-
dc.subject.otherrearrangement excision circles-
dc.subject.otherjuvenile idiopathic arthritis-
dc.subject.otherimmunological self-tolerance-
dc.subject.otherrecent thymic emigrants-
dc.titleThymopoiesis and regulatory T cells in healthy children and adolescents-
dc.rights.holderCopyright HOSPITAL CLINICAS, UNIV SAO PAULO-
dc.subject.wosMedicine, General & Internal-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-, Cristiane:Univ Fed Sao Paulo, Escola Paulista Med, Disciplina Reumatol, Sao Paulo, Brazil-
hcfmusp.publisher.citySAO PAULO-
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Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - FM/MPE
Departamento de Pediatria - FM/MPE

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/ICr
Instituto da Criança - HC/ICr

Artigos e Materiais de Revistas Científicas - LIM/36
LIM/36 - Laboratório de Pediatria Clínica

Artigos e Materiais de Revistas Científicas - LIM/60
LIM/60 - Laboratório de Imunologia Clínica e Alergia

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