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Title: Additional activation of the AR gene may be involved in the development of the castration resistance phenotype in prostate cancer
Authors: ROMAO, P.SOUZA, I. de CamposSILVA, IGUIMARAES, V. RibeiroCAMARGO, J. Alves deSANTOS, G. A. dosVIANA, N. IzabelSROUGI, M.LEITE, K. R. MoreiraREIS, S. T.PIMENTA, R.
Citation: ACTAS UROLOGICAS ESPANOLAS, v.46, n.2, p.78-84, 2022
Abstract: Introduction: Several studies have already shown that changes in the AR gene may be associated with a more aggressive disease phenotype and even castration-resistant prostate cancer. Thus, we investigated cytogenetic and molecular alterations linked to AR. Materials and methods: To evaluate AR methylation, we performed a cytogenetic molecular analysis using fluorescence in situ hybridization that uses specific probes for the AR gene (Xq11.12) and the X chromosome centromere. For AR activity, we performed a qualitative analysis of human androgen receptor activity. To analyze the expression of AR in PC3 and LNCaP cell lines, we used qPCR assays. Results: In the qPCR assay, we found downregulation of AR in the PC-3 cell line compared with the LNCaP. We found the presence of X chromosome polysomy in PC-3 and LNCaP cell lines by FISH assay. In the HUMARA-Q assay, we found two X chromosomes/cell and the activity of both AR in the PC-3 cell line. In LNCaP cells, we found two X chromosomes/cell and methylation of only one AR. Conclusion: Castration-resistant prostate cancer phenotype represents a significant challenge in the setting of urological management. The X chromosomes and AR-linked alterations may contribute to a better understanding of the disease. However, further studies should be performed in an attempt to elucidate as much as possible the role of AR in the castration-resistant prostate cancer phenotype.
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Artigos e Materiais de Revistas Científicas - FM/MCG
Departamento de Cirurgia - FM/MCG

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/55
LIM/55 - Laboratório de Urologia

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar

Artigos e Materiais de Revistas Científicas - ODS/10
ODS/10 - Redução da desigualdade

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