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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorMATTOS, Gabriel J.-
dc.contributor.authorMARCHEAFAVE, Gustavo G.-
dc.contributor.authorROLDAN, William H.-
dc.contributor.authorMATTOS, Miguel J.-
dc.contributor.authorPAULA, Fabiana M. de-
dc.contributor.authorGRYSCHEK, Ronaldo C. B.-
dc.contributor.authorSARTORI, Elen R.-
dc.identifier.citationSENSORS AND ACTUATORS B-CHEMICAL, v.354, article ID 131213, 9p, 2022-
dc.description.abstractStrongyloidiasis is a tropical disease caused by the nematode called Strongyloides stercoralis. An electrochemical immunosensor was efficiently constructed for the diagnosis of this helminthiasis using the larvae epicuticle as the antigen electrostatically immobilized on the surface of a screen-printed electrode, modified with graphene/ ZnOQDs composite. The mechanism of monitoring was based on the changes in the electrochemical parameters of the device due to the antigen-antibody binding on its interface. The immunosensor was characterized using electrochemical impedance spectroscopy and cyclic voltammetry, evaluating the impedimetric/voltammetric biorecognition of the antigen-antibody complex using the redox group K4Fe(CN)6 as the electrochemical probe. This bioelectronic device detected antibodies in positive serum samples based on the voltammetric profile and electrochemical impedance monitoring. Partial least squares-discriminant analysis showed a coefficient of determination of 0.98, indicating that the model can correctly classify samples as positive or negative for strongyloidiasis, based on the voltammetric profile of samples from immunocompetent patients. The analysis of the root mean square error of cross-validation (0.126), the root mean square error of calibration (0.124), and the root mean square error of prediction (0.100) for the latent variable indicate the optimal precision of the model. Based on the receiver operating characteristic curves, the cutoff was determined for the electrochemical impedance measurements, obtaining a 100% correct classification for immunocompetent patients and just 1.25% false-negatives in cases of immunosuppressed patients. The immunosensor presented excellent specificity in the presence of other helminthiases, including ascaridiasis, diphyllobothriasis, himenolepiasis, cysticercosis, and trichuriasis.eng
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [408591/2018-8, 305320/2019-0]-
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)-
dc.description.sponsorshipUniversidade Estadual de Londrina, Hospital das Clinicas - Faculdade de Medicina Universidade de Sao Paulo-
dc.description.sponsorshipInstituto de Medicina Tropical de Sao Paulo-
dc.publisherELSEVIER SCIENCE SAeng
dc.relation.ispartofSensors and Actuators B-Chemical-
dc.subjectElectrochemical immunosensoreng
dc.subjectElectrochemical impedance spectroscopyeng
dc.subjectPrediction modeleng
dc.subjectTropical diseaseseng
dc.subject.otherscreen-printed electrodeseng
dc.subject.otherpartial least-squareseng
dc.subject.otherreduced grapheneeng
dc.titleSerological diagnosis of strongyloidiasis in immunocompetent and immunosuppressed patients based on an electrochemical immunoassay using a flexible device allied to PLS-DA and ROC statistical toolseng
dc.rights.holderCopyright ELSEVIER SCIENCE SAeng
dc.subject.wosChemistry, Analyticaleng
dc.subject.wosInstruments & Instrumentationeng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng, Gabriel J.:Univ Estadual Londrina, Dept Quim, Ctr Ciencias Exatas, Rodovia Celso Garcia Cid,PR 445,Km 380,CxP 100011, BR-86057970 Londrina, PR, Brazil-, Gustavo G.:Univ Estadual Londrina, Dept Quim, Ctr Ciencias Exatas, Rodovia Celso Garcia Cid,PR 445,Km 380,CxP 100011, BR-86057970 Londrina, PR, Brazil-, Miguel J.:Univ Tecnol Fed Parana, Dept Engnh Mat, BR-86036370 Londrina, PR, Brazil-, Elen R.:Univ Estadual Londrina, Dept Quim, Ctr Ciencias Exatas, Rodovia Celso Garcia Cid,PR 445,Km 380,CxP 100011, BR-86057970 Londrina, PR, Brazil-
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Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - LIM/06
LIM/06 - Laboratório de Imunopatologia da Esquistossomose e outras Parasitoses

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