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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | LAZARI, Lucas C. | - |
dc.contributor.author | ZERBINATI, Rodrigo M. | - |
dc.contributor.author | ROSA-FERNANDES, Livia | - |
dc.contributor.author | SANTIAGO, Veronica Feijoli | - |
dc.contributor.author | ROSA, Klaise F. | - |
dc.contributor.author | ANGELI, Claudia B. | - |
dc.contributor.author | SCHWAB, Gabriela | - |
dc.contributor.author | PALMIERI, Michelle | - |
dc.contributor.author | SARMENTO, Dmity J. S. | - |
dc.contributor.author | MARINHO, Claudio R. F. | - |
dc.contributor.author | ALMEIDA, Janete Dias | - |
dc.contributor.author | TO, Kelvin | - |
dc.contributor.author | GIANNECCHINI, Simone | - |
dc.contributor.author | WRENGER, Carsten | - |
dc.contributor.author | SABINO, Ester C. | - |
dc.contributor.author | MARTINHO, Herculano | - |
dc.contributor.author | LINDOSO, Jose A. L. | - |
dc.contributor.author | DURIGON, Edison L. | - |
dc.contributor.author | BRAZ-SILVA, Paulo H. | - |
dc.contributor.author | PALMISANO, Giuseppe | - |
dc.date.accessioned | 2022-04-19T12:59:37Z | - |
dc.date.available | 2022-04-19T12:59:37Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | JOURNAL OF ORAL MICROBIOLOGY, v.14, n.1, article ID 2043651, 12p, 2022 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/45971 | - |
dc.description.abstract | Background The SARS-CoV-2 infections are still imposing a great public health challenge despite the recent developments in vaccines and therapy. Searching for diagnostic and prognostic methods that are fast, low-cost and accurate are essential for disease control and patient recovery. The MALDI-TOF mass spectrometry technique is rapid, low cost and accurate when compared to other MS methods, thus its use is already reported in the literature for various applications, including microorganism identification, diagnosis and prognosis of diseases. Methods Here we developed a prognostic method for COVID-19 using the proteomic profile of saliva samples submitted to MALDI-TOF and machine learning algorithms to train models for COVID-19 severity assessment. Results We achieved an accuracy of 88.5%, specificity of 85% and sensitivity of 91.5% for classification between mild/moderate and severe conditions. When we tested the model performance in an independent dataset, we achieved an accuracy, sensitivity and specificity of 67.18, 52.17 and 75.60% respectively. Conclusion Saliva is already reported to have high inter-sample variation; however, our results demonstrates that this approach has the potential to be a prognostic method for COVID-19. Additionally, the technology used is already available in several clinics, facilitating the implementation of the method. Further investigation using a larger dataset is necessary to consolidate the technique. | eng |
dc.description.sponsorship | Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2018/18257-1, 2018/15549-1, 2020/04923-0, 2015/26722-8, 2017/03966-4, 2018/20468-0, 2021/07490-0, 2020/06409-1] | - |
dc.description.sponsorship | Pro-Reitoria de Pesquisa da Universidade de Sao Paulo [2021.1.10424.1.9] | - |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) [307854/2018-3, 302917/2019-5] | - |
dc.description.sponsorship | Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001] | - |
dc.language.iso | eng | - |
dc.publisher | TAYLOR & FRANCIS LTD | eng |
dc.relation.ispartof | Journal of Oral Microbiology | - |
dc.rights | openAccess | eng |
dc.subject | Saliva | eng |
dc.subject | SARS-CoV-2 | eng |
dc.subject | biomarkers | eng |
dc.subject | proteomics | eng |
dc.subject | prognosis | eng |
dc.subject.other | diagnosis | eng |
dc.title | MALDI-TOF mass spectrometry of saliva samples as a prognostic tool for COVID-19 | eng |
dc.type | article | eng |
dc.rights.holder | Copyright TAYLOR & FRANCIS LTD | eng |
dc.identifier.doi | 10.1080/20002297.2022.2043651 | - |
dc.identifier.pmid | 35251522 | - |
dc.subject.wos | Microbiology | eng |
dc.type.category | original article | eng |
dc.type.version | publishedVersion | eng |
hcfmusp.author.external | LAZARI, Lucas C.:Univ Sao Paulo, Dept Parasitol, GlycoProte Lab, ICB, Sao Paulo, Brazil | - |
hcfmusp.author.external | ROSA-FERNANDES, Livia:Univ Sao Paulo, Dept Parasitol, GlycoProte Lab, ICB, Sao Paulo, Brazil; Univ Sao Paulo, Dept Parasitol, Lab Expt Immunoparasitol, ICB, Sao Paulo, Brazil | - |
hcfmusp.author.external | SANTIAGO, Veronica Feijoli:Univ Sao Paulo, Dept Parasitol, GlycoProte Lab, ICB, Sao Paulo, Brazil | - |
hcfmusp.author.external | ROSA, Klaise F.:Univ Sao Paulo, Dept Parasitol, GlycoProte Lab, ICB, Sao Paulo, Brazil | - |
hcfmusp.author.external | ANGELI, Claudia B.:Univ Sao Paulo, Dept Parasitol, GlycoProte Lab, ICB, Sao Paulo, Brazil | - |
hcfmusp.author.external | PALMIERI, Michelle:Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo, Brazil | - |
hcfmusp.author.external | SARMENTO, Dmity J. S.:Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo, Brazil | - |
hcfmusp.author.external | MARINHO, Claudio R. F.:Univ Sao Paulo, Dept Parasitol, Lab Expt Immunoparasitol, ICB, Sao Paulo, Brazil | - |
hcfmusp.author.external | ALMEIDA, Janete Dias:Sao Paulo State Univ, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, Brazil | - |
hcfmusp.author.external | TO, Kelvin:Univ Hong Kong, Carol Yu Ctr Infect, Dept Microbiol, State Key Lab Emerging Infect Dis,Li KaShing Fac, Hong Kong, Peoples R China | - |
hcfmusp.author.external | GIANNECCHINI, Simone:Univ Florence, Dept Expt & Clin Med, Florence, Italy | - |
hcfmusp.author.external | WRENGER, Carsten:Univ Sao Paulo, Dept Parasitol, Unit Drug Discovery, ICB, Sao Paulo, Brazil | - |
hcfmusp.author.external | MARTINHO, Herculano:Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP, Brazil | - |
hcfmusp.author.external | DURIGON, Edison L.:Univ Sao Paulo, Dept Microbiol, Lab Clin & Mol Virol, ICB, Sao Paulo, Brazil | - |
hcfmusp.author.external | PALMISANO, Giuseppe:Univ Sao Paulo, Dept Parasitol, GlycoProte Lab, ICB, Sao Paulo, Brazil | - |
hcfmusp.description.articlenumber | 2043651 | - |
hcfmusp.description.issue | 1 | - |
hcfmusp.description.volume | 14 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000761614100001 | - |
hcfmusp.origem.id | 2-s2.0-85125922328 | - |
hcfmusp.publisher.city | ABINGDON | eng |
hcfmusp.publisher.country | ENGLAND | eng |
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dc.description.index | PubMed | eng |
dc.identifier.eissn | 2000-2297 | - |
hcfmusp.citation.scopus | 4 | - |
hcfmusp.scopus.lastupdate | 2024-03-08 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - COVID-19 Artigos e Materiais de Revistas Científicas - FM/MIP Artigos e Materiais de Revistas Científicas - LIM/39 Artigos e Materiais de Revistas Científicas - LIM/46 Artigos e Materiais de Revistas Científicas - LIM/49 Artigos e Materiais de Revistas Científicas - LIM/52 Artigos e Materiais de Revistas Científicas - ODS/03 |
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