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Title: Identification of Leishmania species by next generation sequencing of hsp70 gene
Authors: SOUZA, Regina Maia deMARTINS, Roberta Cristina RuedasFRANCO, Lucas Augusto MoysesTUON, Felipe FranciscoOLIVEIRA JUNIOR, Isael Gomes deSILVA, Camila Alves Maia daIMAMURA, RuiAMATO, Valdir Sabbaga
Citation: MOLECULAR AND CELLULAR PROBES, v.61, article ID 101791, 5p, 2022
Abstract: Leishmaniasis is a major public health problem worldwide. Although next generation sequencing technology has been widely used in the diagnosis of infectious diseases, it has been scarcely applied in identification of Leishmania species. The aim of this study was to compare the efficiency of MinIONTM nanopore sequencing and polymerase chain reaction restriction fragment length polymorphism in identifying Leishmania species. Our results showed that the MinIONTM sequencer was able to discriminate reference strains and clinical samples with high sensitivity in a cost and time effective manner without the prior need for culture.
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/32
LIM/32 - Laboratório de Otorrinolaringologia

Artigos e Materiais de Revistas Científicas - LIM/46
LIM/46 - Laboratório de Parasitologia Médica

Artigos e Materiais de Revistas Científicas - LIM/49
LIM/49 - Laboratório de Protozoologia

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