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DC Field | Value | Language |
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dc.contributor | Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP | - |
dc.contributor.author | RODRIGUES, Leandro | - |
dc.contributor.author | REIS, Luciene Machado dos | - |
dc.contributor.author | DENADAI, Rafael | - |
dc.contributor.author | RAPOSO-AMARAL, Cassio Eduardo | - |
dc.contributor.author | ALONSO, Nivaldo | - |
dc.contributor.author | FERREIRA, Marcus Castro | - |
dc.contributor.author | JORGETTI, Vanda | - |
dc.date.accessioned | 2014-04-25T21:49:51Z | - |
dc.date.available | 2014-04-25T21:49:51Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | JOURNAL OF CRANIOFACIAL SURGERY, v.24, n.6, p.1914-1921, 2013 | - |
dc.identifier.issn | 1049-2275 | - |
dc.identifier.uri | https://observatorio.fm.usp.br/handle/OPI/5055 | - |
dc.description.abstract | Background: Extensive bone defects are still a challenge for reconstructive surgery. Allogenic bones can be an alternative with no donor area morbidity and unlimited amount of tissue. Better results can be achieved after allogenic bone preparation and adding a vascular supply, which can be done along with flap prefabrication. The purpose of this study was to evaluate demineralized/lyophilized and deep-frozen allogenic bones used for flap prefabrication and the tissue expression of transforming growth factor beta (TGF-beta) in these bone fragments. Methods: Fifty-six Wistar rat bone diaphyses were prepared and distributed in 4 groups: demineralized/lyophilized (experimental group 1 and control group 2) and deep freezing (experimental group 3 and control group 4). Two bone segments (one of each group) were implanted in rats to prefabricate flaps using superficial epigastric vessels (experimental groups) or only transferred as grafts (control groups). These fragments remained in their respective inguinal regions until the death that occurred at 2, 4, and 6 weeks after the operation. Semiquantitative histologic (tetracycline marking, cortical resorption, number of giant cells, and vascularization) and histomorphometrical quantitative (osteoid thickness, cortical thickness, and fibrosis thickness) analyses were performed. Transforming growth factor beta immunohistochemistry staining was also performed. Results: Group 1 fragments presented an osteoid matrix on their external surface in all periods. Cartilage formation and mineralization areas were also noticed. These findings were not observed in group 3 fragments. Group 1 had more mineralization and double tetracycline marks, which were almost not seen in group 3. Cortical resorption and the number of giant cells were greater in group 3 in all periods. Vascularization and fibrosis thickness were similar in both experimental groups. Group 1 had more intense TGF-beta staining within 2 weeks of study. Nevertheless, from 4 weeks onward, group 3 presented statistically significant stronger staining. Conclusions: Although there are some differences between the preparation methods of allogenic bone, it is possible to prefabricate flaps with demineralized/lyophilized and deep-frozen bones. | - |
dc.language.iso | eng | - |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | - |
dc.relation.ispartof | Journal of Craniofacial Surgery | - |
dc.rights | restrictedAccess | - |
dc.subject | Allogenic bone | - |
dc.subject | prefabricated flaps | - |
dc.subject | preparation | - |
dc.subject | rats | - |
dc.subject | TGF-beta | - |
dc.subject.other | craniofacial surgery | - |
dc.subject.other | rat model | - |
dc.subject.other | allografts | - |
dc.subject.other | transplantation | - |
dc.subject.other | reconstruction | - |
dc.subject.other | implants | - |
dc.subject.other | defects | - |
dc.subject.other | grafts | - |
dc.subject.other | strategies | - |
dc.subject.other | experience | - |
dc.title | Prefabricated Bone Flap: An Experimental Study Comparing Deep-Frozen and Lyophilized-Demineralized Allogenic Bones and Tissue Expression of Transforming Growth Factor beta | - |
dc.type | article | - |
dc.rights.holder | Copyright LIPPINCOTT WILLIAMS & WILKINS | - |
dc.identifier.doi | 10.1097/SCS.0b013e3182a41be2 | - |
dc.identifier.pmid | 24220373 | - |
dc.subject.wos | Surgery | - |
dc.type.category | original article | - |
dc.type.version | publishedVersion | - |
hcfmusp.author.external | RODRIGUES, Leandro:Univ Sao Paulo, Fac Med, Div Plast Surg, Dept Surg, Sao Paulo, Brazil | - |
hcfmusp.author.external | DENADAI, Rafael:SOBRAPAR Hosp, Inst Plast & Craniofacial Surg, Sao Paulo, Brazil | - |
hcfmusp.author.external | RAPOSO-AMARAL, Cassio Eduardo:SOBRAPAR Hosp, Inst Plast & Craniofacial Surg, Sao Paulo, Brazil | - |
hcfmusp.description.beginpage | 1914 | - |
hcfmusp.description.endpage | 1921 | - |
hcfmusp.description.issue | 6 | - |
hcfmusp.description.volume | 24 | - |
hcfmusp.origem | WOS | - |
hcfmusp.origem.id | WOS:000330354500042 | - |
hcfmusp.origem.id | 2-s2.0-84888379468 | - |
hcfmusp.publisher.city | PHILADELPHIA | - |
hcfmusp.publisher.country | USA | - |
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dc.description.index | MEDLINE | - |
dc.identifier.eissn | 1536-3732 | - |
hcfmusp.citation.scopus | 2 | - |
hcfmusp.scopus.lastupdate | 2024-03-29 | - |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MCG Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/04 Artigos e Materiais de Revistas Científicas - LIM/16 |
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