Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/50672
Title: SARS-CoV-2 infection impacts carbon metabolism and depends on glutamine for replication in Syrian hamster astrocytes
Authors: OLIVEIRA, Lilian Gomes deANGELO, Yan de SouzaYAMAMOTO, PedroCARREGARI, Victor CorasollaCRUNFLI, FernandaREIS-DE-OLIVEIRA, GuilhermeCOSTA, LiciaVENDRAMINI, Pedro HenriqueDUQUE, Erica AlmeidaSANTOS, Nilton Barreto dosFIRMINO, Egidi MayaraPAIVA, Isadora MarquesALMEIDA, Glaucia MariaSEBOLLELA, AdrianoPOLONIO, Carolina ManganeliZANLUQUI, Nagela GhabdanOLIVEIRA, Marilia Garcia deSILVA, Patrick daDAVANZO, Gustavo GastaoAYUPE, Marina CacadorSALGADO, Caio LoureiroSOUZA FILHO, Antonio Francisco deARAUJO, Marcelo Valdemir deSILVA-PEREIRA, Taiana TainaCAMPOS, Angelica Cristine de AlmeidaGOES, Luiz Gustavo BentimCUNHA, Marielton dos PassosCALDINI, Elia GarciaLIMA, Maria Regina D'ImperioFONSECA, Denise MoraisGUIMARAES, Ana Marcia de SaMINOPRIO, Paola CamargoMUNHOZ, Carolina DemarchiMORI, Claudia Madalena CabreraMORAES-VIEIRA, Pedro ManoelCUNHA, Thiago MattarMARTINS-DE-SOUZA, DanielPERON, Jean Pierre Schatzmann
Citation: JOURNAL OF NEUROCHEMISTRY, v.163, n.2, p.113-132, 2022
Abstract: COVID-19 causes more than million deaths worldwide. Although much is understood about the immunopathogenesis of the lung disease, a lot remains to be known on the neurological impact of COVID-19. Here, we evaluated immunometabolic changes using astrocytes in vitro and dissected brain areas of SARS-CoV-2 infected Syrian hamsters. We show that SARS-CoV-2 alters proteins of carbon metabolism, glycolysis, and synaptic transmission, many of which are altered in neurological diseases. Real-time respirometry evidenced hyperactivation of glycolysis, further confirmed by metabolomics, with intense consumption of glucose, pyruvate, glutamine, and alpha ketoglutarate. Consistent with glutamine reduction, the blockade of glutaminolysis impaired viral replication and inflammatory response in vitro. SARS-CoV-2 was detected in vivo in hippocampus, cortex, and olfactory bulb of intranasally infected animals. Our data evidence an imbalance in important metabolic molecules and neurotransmitters in infected astrocytes. We suggest this may correlate with the neurological impairment observed during COVID-19, as memory loss, confusion, and cognitive impairment.
Appears in Collections:

Artigos e Materiais de Revistas Científicas - COVID-19
COVID-19

Artigos e Materiais de Revistas Científicas - FM/MPT
Departamento de Patologia - FM/MPT

Artigos e Materiais de Revistas Científicas - LIM/59
LIM/59 - Laboratório de Biologia Celular

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar


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