Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/5097
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorZANONI, Fernando Luiz-
dc.contributor.authorCRUZ, Jose Walber Miranda Costa-
dc.contributor.authorMARTINS, Joilson Oliveira-
dc.contributor.authorBENABOU, Simon-
dc.contributor.authorGRECO, Karin Vicente-
dc.contributor.authorMORENO, Ana Carolina Ramos-
dc.contributor.authorMARTINEZ, Marina Baquerizo-
dc.contributor.authorCALDERARO, Franco Ferraro-
dc.contributor.authorSILVA, Mauricio Rocha e-
dc.contributor.authorSANNOMIYA, Paulina-
dc.date.accessioned2014-04-25T21:50:37Z-
dc.date.available2014-04-25T21:50:37Z-
dc.date.issued2013-
dc.identifier.citationSHOCK, v.40, n.1, p.35-44, 2013-
dc.identifier.issn1073-2322-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/5097-
dc.description.abstractWe examined the effects of hypertonic saline (HS) on inflammatory, metabolic variables, and bacterial translocation (BT) in rats submitted to intestinal obstruction and ischemia (IO). Male Wistar rats were submitted to IO and treated, 2 h thereafter, with lactated Ringer's (LR) (4 mL/kg per 5 min, i.v.) or HS (7.5% NaCl, 4 mL/kg per 5 min, i.v.). Twenty-four hours after IO, rats were also submitted to enterectomy/enteroanastomosis to resection of necrotized small bowel. Leukocyte-endothelial interactions were investigated by intravital microscopy and the expression of P-selectin and intercellular adhesion molecule 1 by immunohistochemistry. Bacterial cultures of mesenteric lymph nodes, liver, spleen, and blood were used to evaluate BT. Levels of chemokines (cytokine-induced neutrophil chemoattractants 1 and 2), insulin, and corticosterone were determined by enzyme-linked immunosorbent assay. Intestinal histology, serum urea and creatinine levels, and hepatic enzymes activities were performed to evaluate local and remote damage. Relative to IO and LR-treated rats, which exhibited increases in the number of rolling (1.5-fold), adhered (3.5-fold) and migrated (9.0-fold) leukocytes, and increased expression of P-selectin (3-fold) and intercellular adhesion molecule 1 (3-fold) on mesenteric microcirculation, treatment with HS followed by enterectomy reduced leukocyte-endothelial interactions and expression of both adhesion molecules to values attained in sham rats. Serum chemokines were normalized after treatment with both solutions followed by enterectomy. Hypertonic saline-treated rats demonstrated a significant reduction in BT to 50% in liver and spleen samples and bacteremia (14%), compared with 82% of BT in liver and spleen samples of IO and LR-treated rats and bacteremia (57%). Local intestinal damage was attenuated, and renal and hepatic function preserved by treatment with HS followed by enterectomy. Survival rate increased to 86% up to 15 days. Data presented suggest that HS solution followed by enterectomy reduces mesenteric microcirculatory dysfunctions and BT, attenuating local and remote damage in a model of strangulated small bowel obstruction.-
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [04/15964-6]-
dc.description.sponsorshipCenters of Excellence Program (PRONEX-CNPq/FAPERJ) [66.11251998-2]-
dc.language.isoeng-
dc.publisherLIPPINCOTT WILLIAMS & WILKINS-
dc.relation.ispartofShock-
dc.rightsrestrictedAccess-
dc.subjectGut-
dc.subjectsepsis-
dc.subjectmicrocirculation-
dc.subjectleukocyte-endothelial interactions-
dc.subjectchemokines-
dc.subject.othercritically-ill patients-
dc.subject.otherischemia-reperfusion injury-
dc.subject.otherhemorrhagic-shock-
dc.subject.otherseptic shock-
dc.subject.otherintestinal microcirculation-
dc.subject.othercecal ligation/puncture-
dc.subject.otherleukocyte behavior-
dc.subject.otherprostaglandin e(2)-
dc.subject.otherorgan dysfunction-
dc.subject.othersevere sepsis-
dc.titleHYPERTONIC SALINE SOLUTION REDUCES MESENTERIC MICROCIRCULATORY DYSFUNCTIONS AND BACTERIAL TRANSLOCATION IN A RAT MODEL OF STRANGULATED SMALL BOWEL OBSTRUCTION-
dc.typearticle-
dc.rights.holderCopyright LIPPINCOTT WILLIAMS & WILKINS-
dc.identifier.doi10.1097/SHK.0b013e318299d3fa-
dc.identifier.pmid23644577-
dc.subject.wosCritical Care Medicine-
dc.subject.wosHematology-
dc.subject.wosSurgery-
dc.subject.wosPeripheral Vascular Disease-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-
hcfmusp.author.externalMARTINS, Joilson Oliveira:Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo, Brazil-
hcfmusp.author.externalGRECO, Karin Vicente:St Bartholomews & Royal London Sch Med, William Harvey Res Inst, London, England-
hcfmusp.author.externalMORENO, Ana Carolina Ramos:Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo, Brazil-
hcfmusp.author.externalMARTINEZ, Marina Baquerizo:Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo, Brazil-
hcfmusp.author.externalCALDERARO, Franco Ferraro:Univ Guarulhos, Small Anim Vet Hosp, Sao Paulo, Brazil-
hcfmusp.description.beginpage35-
hcfmusp.description.endpage44-
hcfmusp.description.issue1-
hcfmusp.description.volume40-
hcfmusp.origemWOS-
hcfmusp.origem.id2-s2.0-84880078196-
hcfmusp.origem.idWOS:000330243800007-
hcfmusp.publisher.cityPHILADELPHIA-
hcfmusp.publisher.countryUSA-
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dc.description.indexMEDLINE-
dc.identifier.eissn1540-0514-
hcfmusp.remissive.sponsorshipCNPq-
hcfmusp.remissive.sponsorshipFAPESP-
hcfmusp.remissive.sponsorshipCNPq-
hcfmusp.remissive.sponsorshipFAPESP-
hcfmusp.remissive.sponsorshipFAPERJ-
hcfmusp.citation.scopus10-
hcfmusp.scopus.lastupdate2022-06-24-
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Artigos e Materiais de Revistas Científicas - FM/MCP
Departamento de Cardio-Pneumologia - FM/MCP

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Instituto Central - HC/ICHC

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Artigos e Materiais de Revistas Científicas - LIM/11
LIM/11 - Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação


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