https://observatorio.fm.usp.br/handle/OPI/5142
Title: | Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers |
Authors: | PEREIRA, Natalli Zanete; CARDOSO, Elaine Cristina; OLIVEIRA, Luanda Mara da Silva; LIMA, Josenilson Feitosa de; BRANCO, Anna Claudia Calvielli Castelo; RUOCCO, Rosa Maria de Souza Aveiro; ZUGAIB, Marcelo; OLIVEIRA FILHO, Joao Bosco de; DUARTE, Alberto Jose da Silva; SATO, Maria Notomi |
Citation: | PLOS ONE, v.8, n.12, article ID e84917, 11p, 2013 |
Abstract: | Programs for the prevention of mother-to-child transmission of HIV have reduced the transmission rate of perinatal HIV infection and have thereby increased the number of HIV-exposed uninfected (HEU) infants. Natural immunity to HIV-1 infection in both mothers and newborns needs to be further explored. In this study, we compared the expression of antiviral restricting factors in HIV-infected pregnant mothers treated with antiretroviral therapy (ART) in pregnancy (n=23) and in cord blood (CB) (n=16), placental tissues (n=10-13) and colostrum (n=5-6) samples and compared them to expression in samples from uninfected (UN) pregnant mothers (n=21). Mononuclear cells (MNCs) were prepared from maternal and CB samples following deliveries by cesarean section. Maternal (decidua) and fetal (chorionic villus) placental tissues were obtained, and colostrum was collected 24 h after delivery. The mRNA and protein expression levels of antiviral factors were then evaluated. We observed a significant increase in the mRNA expression levels of antiviral factors in MNCs from HIV-infected mothers and CB, including the apolipoprotein B mRNA-editing enzyme 3G (A3G), A3F, tripartite motif family-5 alpha (TRIM-5 alpha), TRIM-22, myxovirus resistance protein A (MxA), stimulator of interferon (IFN) genes (STING) and IFN-beta, compared with the levels detected in uninfected (UN) mother-CB pairs. Moreover, A3G transcript and protein levels and alpha-defensin transcript levels were decreased in the decidua of HIV-infected mothers. Decreased TRIM-5 alpha protein levels in the villi and increased STING mRNA expression in both placental tissues were also observed in HIV-infected mothers compared with uninfected (UN) mothers. Additionally, colostrum cells from infected mothers showed increased tetherin and IFN-beta mRNA levels and CXCL9 protein levels. The data presented here indicate that antiviral restricting factor expression can be induced in utero in HIV-infected mothers. Future studies are warranted to determine whether this upregulation of antiviral factors during the perinatal period has a protective effect against HIV-1 infection. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MDT Artigos e Materiais de Revistas Científicas - FM/MOG Artigos e Materiais de Revistas Científicas - FM/MPT Artigos e Materiais de Revistas Científicas - HC/ICHC Artigos e Materiais de Revistas Científicas - LIM/56 Artigos e Materiais de Revistas Científicas - LIM/57 Artigos e Materiais de Revistas Científicas - ODS/03 Artigos e Materiais de Revistas Científicas - ODS/05 |
File | Description | Size | Format | |
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art_OLIVEIRA_Upregulation_of_Innate_Antiviral_Restricting_Factor_Expression_in_2013.PDF | publishedVersion (English) | 1.19 MB | Adobe PDF | View/Open |
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