Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/523
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorSAAD, Paulo Fernandes-
dc.contributor.authorSAAD, Karen Ruggeri-
dc.contributor.authorOLIVEIRA FILHO, Luiz Dantas de-
dc.contributor.authorFERREIRA, Sueli Gomes-
dc.contributor.authorKOIKE, Marcia Kiyomi-
dc.contributor.authorMONTERO, Edna Frasson de Souza-
dc.date.accessioned2013-07-30T14:42:00Z-
dc.date.available2013-07-30T14:42:00Z-
dc.date.issued2012-
dc.identifier.citationACTA CIRURGICA BRASILEIRA, v.27, n.8, p.561-565, 2012-
dc.identifier.issn0102-8650-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/523-
dc.description.abstractPURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.-
dc.description.abstractOBJETIVO: Avaliar o efeito da N-acetilcisteína (NAC) combinada ao fluido de reposição volêmica na morte celular pulmonar de ratos submetidos ao choque hemorrágico (CH) controlado. MÉTODOS: Duas artérias (cálculo da PAM e exsanguinação) e uma veia (tratamentos) foram cateterizadas em 22 ratos anestesiados. Dois grupos de ratos machos albinos foram induzidos ao CH controlado com PAM de 35mmHg por 60 min. Após este período, o grupo RL foi ressuscitado com Ringer lactato e o grupo RL+NAC foi ressuscitado com Ringer lactato associado com 150mg/Kg de NAC. O grupo controle sofreu somente o procedimento cirúrgico de cateterização. Os animais sofreram eutanásia após 120 min. da ressuscitação. Amostras de tecido pulmonar foram coletadas para histopatologia, TUNEL e a imuno-expressão da caspase 3. RESULTADOS: RL apresentou maior número de células marcadas pelo TUNEL do que RL+NAC, porém sem alteração na expressão da caspase 3 em nenhum dos grupos estudados. CONCLUSÃO: A N-acetilcisteína teve um papel protetor na morte celular em modelo de choque hemorrágico controlado.-
dc.description.sponsorshipNational Council of Technological and Scientific Development (CNPq)-
dc.language.isoeng-
dc.publisherACTA CIRURGICA BRASILEIRA-
dc.relation.ispartofActa Cirurgica Brasileira-
dc.rightsopenAccess-
dc.subjectAcetylcysteine-
dc.subjectLung-
dc.subjectShock, Hemorrhagic-
dc.subjectCell Death-
dc.subjectRats-
dc.subjectAcetilcisteína-
dc.subjectPulmão-
dc.subjectChoque Hemorrágico-
dc.subjectMorte Celular-
dc.subjectRatos-
dc.subject.otheracute lung injury-
dc.subject.otherfluid resuscitation-
dc.subject.otheroncotic necrosis-
dc.subject.otherapoptosis-
dc.subject.othergeneration-
dc.subject.otherliver-
dc.titleEffect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats-
dc.title.alternativeEfeito da N-acetilcisteína na morte celular pulmonar em um modelo de choque hemorrágico controlado em ratos-
dc.typearticle-
dc.rights.holderCopyright ACTA CIRURGICA BRASILEIRA-
dc.identifier.doi10.1590/S0102-86502012000800008-
dc.identifier.pmid22850708-
dc.subject.wosSurgery-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-
hcfmusp.author.externalSAAD, Paulo Fernandes:Univ Fed Sao Paulo, Postgrad Program Interdisciplinary Surg Sci, Paulista Med Sch, Sao Paulo, Brazil-
hcfmusp.author.externalSAAD, Karen Ruggeri:Sao Francisco Valley Fed Univ UNIVASF, Fac Med, Petrolina, PE, Brazil-
hcfmusp.author.externalOLIVEIRA FILHO, Luiz Dantas de:Fundacao Univ Fed Vale Sao Francisco, Fac Med, Petrolina, PE, Brazil-
hcfmusp.description.beginpage561-
hcfmusp.description.endpage565-
hcfmusp.description.issue8-
hcfmusp.description.volume27-
hcfmusp.origemWOS-
hcfmusp.origem.id2-s2.0-84865626248-
hcfmusp.origem.idWOS:000306972600008-
hcfmusp.origem.idSCIELO:S0102-86502012000800008-
hcfmusp.publisher.citySAO PAULO-
hcfmusp.publisher.countryBRAZIL-
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dc.description.indexMEDLINE-
hcfmusp.remissive.sponsorshipCNPq-
hcfmusp.lim.ref2012-
hcfmusp.citation.scopus5-
hcfmusp.scopus.lastupdate2022-06-24-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MCG
Departamento de Cirurgia - FM/MCG

Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - LIM/51
LIM/51 - Laboratório de Emergências Clínicas

Artigos e Materiais de Revistas Científicas - LIM/62
LIM/62 - Laboratório de Fisiopatologia Cirúrgica


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