Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/56107
Title: Characterization of Torquetenovirus in amniotic fluid at the time of <i>in utero</i> fetal surgery: correlation with early premature delivery and respiratory distress
Authors: TOZETTO-MENDOZA, Tania ReginaDA-COSTA, A. CharlysMORON, Antonio F.LEAL, ElcioLIMA, Silvia HelenaFERREIRA, Noely EvangelistaHONORATO, LaylaPAIAO, Heuder Gustavo OliveiraFREIRE, Wilton SantosMENDES-CORREA, Maria CassiaWITKIN, Steven S.
Citation: FRONTIERS IN MEDICINE, v.10, article ID 1161091, 8p, 2023
Abstract: Torquetenovirus (TTV) is a commensal virus present in many healthy individuals. Although considered to be non-pathogenic, its presence and titer have been shown to be indicative of altered immune status in individuals with chronic infections or following allogeneic transplantations. We evaluated if TTV was present in amniotic fluid (AF) at the time of in utero surgery to correct a fetal neurological defect, and whether its detection was predictive of adverse post-surgical parameters. AF was collected from 27 women by needle aspiration prior to a uterine incision. TTV titer in the AF was measured by isolation of viral DNA followed by gene amplification and analysis. The TTV genomes were further characterized and sequenced by metagenomics. Pregnancy outcome parameters were subsequently obtained by chart review. Three of the AFs (11.1%) were positive for TTV at 3.36, 4.16, and 4.19 log(10) copies/mL. Analysis of their genomes revealed DNA sequences similar to previously identified TTV isolates. Mean gestational age at delivery was >2 weeks earlier (32.5 vs. 34.6 weeks) and the prevalence of respiratory distress was greater (100% vs. 20.8%) in the TTV-positive pregnancies. TTV detection in AF prior to intrauterine surgery may indicate elevated post-surgical risk for earlier delivery and newborn respiratory distress.
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - IMT
Instituto de Medicina Tropical - IMT

Artigos e Materiais de Revistas Científicas - LIM/46
LIM/46 - Laboratório de Parasitologia Médica

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia


Files in This Item:
File Description SizeFormat 
art_TOZETTO-MENDOZA_Characterization_of_Torquetenovirus_in_amniotic_fluid_at_the_2023.PDFpublishedVersion (English)1.72 MBAdobe PDFThumbnail
View/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.