Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/56318
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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorCAMARGO, Carlos Henrique-
dc.contributor.authorYAMADA, Amanda Yaeko-
dc.contributor.authorSOUZA, Andreia Rodrigues de-
dc.contributor.authorCUNHA, Marcos Paulo Vieira-
dc.contributor.authorFERRARO, Pedro Smith Pereira-
dc.contributor.authorSACCHI, Claudio Tavares-
dc.contributor.authorSANTOS, Marlon Benedito dos-
dc.contributor.authorCAMPOS, Karoline Rodrigues-
dc.contributor.authorTIBA-CASAS, Monique Ribeiro-
dc.contributor.authorFREIRE, Maristela Pinheiro-
dc.contributor.authorBARRETTI, Pasqual-
dc.date.accessioned2023-10-30T14:56:25Z-
dc.date.available2023-10-30T14:56:25Z-
dc.date.issued2023-
dc.identifier.citationSCIENTIFIC REPORTS, v.13, n.1, article ID 14603, 9p, 2023-
dc.identifier.issn2045-2322-
dc.identifier.urihttps://observatorio.fm.usp.br/handle/OPI/56318-
dc.description.abstractCarbapenem-resistant Klebsiella pneumoniae (CRKP) are highly disseminated worldwide, and isolates co-resistant to other antimicrobial agents pose a threat to effective antimicrobial therapy. Therefore, evaluation of novel antimicrobial drugs is needed to identify potential treatments with better outcomes. We evaluated the in vitro activity of novel antimicrobial drugs/combinations against 97 KPC-producing Klebsiella pneumoniae isolates recovered from different hospitals in Brazil during 2021-2022. Clonality, resistance and virulence genes were detected by whole-genome sequencing. The majority of the isolates (54.6%) were classified as extensively drug resistant or multidrug resistant (44.3%); one isolate showed a pandrug resistance phenotype. The most active antimicrobial agents were meropenem-vaborbactam, cefiderocol, and ceftazidime-avibactam, with sensitivities higher than 90%; resistance to ceftazidime-avibactam was associated with KPC-33 or KPC-44 variants. Colistin and polymyxin B were active against 58.6% of the isolates. The 97 isolates were distributed into 17 different sequence types, with a predominance of ST11 (37.4%). Although high in vitro susceptibility rates were detected for meropenem-vaborbactam and cefiderocol, only ceftazidime-avibactam is currently available in Brazil. Our findings showed limited susceptibility to antimicrobial drugs employed for infection treatment of carbapenem-resistant K. pneumoniae, underscoring the urgent need for stringent policies for antimicrobial stewardship to preserve the activity of such drugs.eng
dc.description.sponsorshipPfizer/Wyeth-
dc.description.sponsorshipInvestigator Sponsored Research-
dc.description.sponsorshipSao Paulo Research Foundation FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) [2020/06157-2, 2017/50333-7, 2018/21192-9]-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [402158/2021-0]-
dc.description.sponsorshipFESIMA (Fundo Especial de Saude para Imunizacao em Massa e Controle de Doencas)-
dc.language.isoeng-
dc.publisherNATURE PORTFOLIOeng
dc.relation.ispartofScientific Reports-
dc.rightsopenAccesseng
dc.subject.othersao-pauloeng
dc.subject.otherresistanceeng
dc.subject.otherenterobacteriaceaeeng
dc.subject.otherinfectionseng
dc.subject.otheremergenceeng
dc.subject.otherplasmidseng
dc.subject.othercomplexeng
dc.subject.othercloneseng
dc.titleGenomic analysis and antimicrobial activity of β-lactam/β-lactamase inhibitors and other agents against KPC-producing <i>Klebsiella pneumoniae</i> clinical isolates from Brazilian hospitalseng
dc.typearticleeng
dc.rights.holderCopyright NATURE PORTFOLIOeng
dc.identifier.doi10.1038/s41598-023-41903-x-
dc.identifier.pmid37670032-
dc.subject.wosMultidisciplinary Scienceseng
dc.type.categoryoriginal articleeng
dc.type.versionpublishedVersioneng
hcfmusp.author.externalSOUZA, Andreia Rodrigues de:Adolfo Lutz Inst, Ctr Bacteriol, Ave Dr Arnaldo 351,9 Andar, BR-01246902 Sao Paulo, SP, Brazil-
hcfmusp.author.externalCUNHA, Marcos Paulo Vieira:Adolfo Lutz Inst, Ctr Bacteriol, Ave Dr Arnaldo 351,9 Andar, BR-01246902 Sao Paulo, SP, Brazil-
hcfmusp.author.externalFERRARO, Pedro Smith Pereira:Adolfo Lutz Inst, Ctr Bacteriol, Ave Dr Arnaldo 351,9 Andar, BR-01246902 Sao Paulo, SP, Brazil-
hcfmusp.author.externalSACCHI, Claudio Tavares:Adolfo Lutz Inst, Lab Estrateg, Ave Dr Arnaldo 351,10 Andar, BR-01246902 Sao Paulo, Brazil-
hcfmusp.author.externalSANTOS, Marlon Benedito dos:Adolfo Lutz Inst, Lab Estrateg, Ave Dr Arnaldo 351,10 Andar, BR-01246902 Sao Paulo, Brazil-
hcfmusp.author.externalCAMPOS, Karoline Rodrigues:Adolfo Lutz Inst, Lab Estrateg, Ave Dr Arnaldo 351,10 Andar, BR-01246902 Sao Paulo, Brazil-
hcfmusp.author.externalTIBA-CASAS, Monique Ribeiro:Adolfo Lutz Inst, Ctr Bacteriol, Ave Dr Arnaldo 351,9 Andar, BR-01246902 Sao Paulo, SP, Brazil-
hcfmusp.author.externalBARRETTI, Pasqual:Univ Estadual Paulista, Fac Med Botucatu, Ave Prof Montenegro,S-N, BR-18618687 Botucatu, Brazil-
hcfmusp.description.articlenumber14603-
hcfmusp.description.issue1-
hcfmusp.description.volume13-
hcfmusp.origemWOS-
hcfmusp.origem.idWOS:001062861100034-
hcfmusp.origem.id2-s2.0-85169756525-
hcfmusp.publisher.cityBERLINeng
hcfmusp.publisher.countryGERMANYeng
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dc.description.indexPubMed-
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dc.description.indexScopus-
hcfmusp.citation.scopus0-
hcfmusp.scopus.lastupdate2024-04-12-
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