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https://observatorio.fm.usp.br/handle/OPI/571
Title: | The spider acylpolyamine Mygalin is a potent modulator of innate immune responses |
Authors: | MAFRA, Diego Gabriel; SILVA JR., Pedro Ismael da; GALHARDO, Cynthia Soares; NASSAR, Rafael; DAFFRE, Sirlei; SATO, Maria N.; BORGES, Monamaris M. |
Citation: | CELLULAR IMMUNOLOGY, v.275, n.1-2, p.5-11, 2012 |
Abstract: | Mygalin is an antibacterial molecule isolated froth the hemocytes of the spider Acanthoscurria gomesiana. It was identified as bis-acylpolyamine spermidine. We evaluated the modulator effects of synthetic Mygalin in the innate immune response. We demonstrate that Mygalin induces IFN-gamma synthesis by splenocytes increasing the nitrite secretion by splenocytes and macrophages. A specific inhibitor of iNOS abrogated Mygalin-induced nitrite production in macrophages independent of IFN-gamma activation. In addition, Mygalin-activated macrophages produced TNF-alpha but not IL-1 beta, demonstrating that Mygalin does not act directly on the inflammasome. Furthermore, this compound did not affect spontaneous or Concanavalin A-induced proliferative responses by murine splenocytes and did not induce IL-5 or apoptosis of splenocytes or bone marrow-derived macrophages. These data provide evidence that Mygalin modulates the innate immune response by inducing IFN-gamma and NO synthesis. The combined immune regulatory and antibacterial qualities of Mygalin should be explored as a strategy to enhance immune responses in infection. |
Appears in Collections: | Artigos e Materiais de Revistas Científicas - FM/MDT Artigos e Materiais de Revistas Científicas - LIM/56 |
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