Please use this identifier to cite or link to this item: https://observatorio.fm.usp.br/handle/OPI/745
Title: Pilot Studies for Development of an HIV Subtype Panel for Surveillance of Global Diversity
Authors: MANAK, MarkSINA, SilvanaANEKELLA, BharathiHEWLETT, IndiraSANDERS-BUELL, EricRAGUPATHY, ViswanathKIM, JeromeVERMEULEN, MarionSTRAMER, Susan L.SABINO, EsterGRABARCZYK, PiotrMICHAEL, NelsonPEEL, SheilaGARRETT, PatriciaTOVANABUTRA, SodsaiBUSCH, Michael P.SCHITO, Marco
Citation: AIDS RESEARCH AND HUMAN RETROVIRUSES, v.28, n.6, p.594-606, 2012
Abstract: The continued global spread and evolution of HIV diversity pose significant challenges to diagnostics and vaccine strategies. NIAID partnered with the FDA, WRAIR, academia, and industry to form a Viral Panel Working Group to design and prepare a panel of well-characterized current and diverse HIV isolates. Plasma samples that had screened positive for HIV infection and had evidence of recently acquired infection were donated by blood centers in North and South America, Europe, and Africa. A total of 80 plasma samples were tested by quantitative nucleic acid tests, p24 antigen, EIA, and Western blot to assign a Fiebig stage indicative of approximate time from initial infection. Evaluation of viral load using FDA-cleared assays showed excellent concordance when subtype B virus was tested, but lower correlations for subtype C. Plasma samples were cocultivated with phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMCs) from normal donors to generate 30 viral isolates (50-80% success rate for samples with viral load >10,000 copies/ml), which were then expanded to 10(7)-10(9) virus copies per ml. Analysis of env sequences showed that sequences derived from cultured PBMCs were not distinguishable from those obtained from the original plasma. The pilot collection includes 30 isolates representing subtypes B, C, B/F, CRF04_cpx, and CRF02_AG. These studies will serve as a basis for the development of a comprehensive panel of highly characterized viral isolates that reflects the current dynamic and complex HIV epidemic, and will be made available through the External Quality Assurance Program Oversight Laboratory (EQAPOL).
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - LIM/52
LIM/52 - Laboratório de Virologia


Files in This Item:
File Description SizeFormat 
art_SABINO_Pilot_Studies_for_Development_of_an_HIV_Subtype_2012.PDF
  Restricted Access
publishedVersion (English)547.28 kBAdobe PDFView/Open Request a copy

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.