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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorTORRICELLI, Andre Augusto Miranda-
dc.contributor.authorMATSUDA, Monique-
dc.contributor.authorNOVAES, Priscila-
dc.contributor.authorBRAGA, Alfesio Luiz Ferreira-
dc.contributor.authorSALDIVA, Paulo Hilario Nascimento-
dc.contributor.authorALVES, Milton Ruiz-
dc.contributor.authorMONTEIRO, Mario Luiz Ribeiro-
dc.identifier.citationENVIRONMENTAL RESEARCH, v.131, p.59-63, 2014-
dc.description.abstractPurpose: To quantify ocular symptoms, goblet cells (GC) and mucin 5AC (MUC5AC) gene expression on the conjunctiva of healthy subjects exposed to ambient levels of traffic-derived air pollution and to estimate its correlation with NO2 and particulate matter smaller than 2.5 mu m (PM2.5) levels. Methods: Twenty-one taxi drivers or traffic controllers were assessed with the Ocular Surface Disease Index (OSDI) questionnaire and conjunctival impression cytology. MUC5AC mRNA levels were determined based on the cytology of the right eye, and GC density was assessed based on the cytology of the left eye. Mean individual levels of 24-h NO2 and PM2.5 exposure were assessed the day before examination. Possible associations between NO2 or PM2.5 levels, OSDI scores, GC densities and MUC5AC mRNA levels were verified. Results: The subjects were exposed to mean PM2.5 levels of 35 +/- 12 mu g/m(3) and mean NO2 levels of 189 +/- 47 mu g/m(3). OSDI scores were low (7.4 +/- 8) and GC densities were 521 +/- 257 and 782 +/- 322 cell/mm(2) on the bulbar and tarsal conjunctivas, respectively. The mean GC-derived MUC5AC mRNA expression was 14 +/- 7 fM/mu g of total RNA. A significant and positive correlation was observed between MUC5AC mRNA levels and tarsal GC density (p=0.018). A trend toward association between PM2.5 levels and tarsal GC cell density (p=0.052) was found. Conclusion: Exposure to ambient levels of air pollution impacts conjunctival GC density. An increase in MUC5AC mRNA levels may be part of an adaptive ocular surface response to long-term exposure to air pollution.-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)-
dc.description.sponsorshipCNPq Brasilia, Brazil [306487/2011-0, 555223/2006-0]-
dc.description.sponsorshipCAPES, Brasilia, Brazil [8923-12-4]-
dc.relation.ispartofEnvironmental Research-
dc.subjectAir pollution-
dc.subjectOcular surface-
dc.subjectGoblet cell-
dc.subject.otherimpression cytology-
dc.subject.otherdisease index-
dc.subject.otherdry eye-
dc.titleEffects of ambient levels of traffic-derived air pollution on the ocular surface: Analysis of symptoms, conjunctival goblet cell count and mucin 5AC gene expression-
dc.subject.wosEnvironmental Sciences-
dc.subject.wosPublic, Environmental & Occupational Health-
dc.type.categoryoriginal article-
hcfmusp.publisher.citySAN DIEGO-
hcfmusp.relation.referenceAragona P, 1996, EYE, V10, P82-
hcfmusp.relation.referenceArgueso P, 2002, INVEST OPHTH VIS SCI, V43, P1004-
hcfmusp.relation.referenceBasbaum C, 1999, AM J RESP CRIT CARE, V160, pS44-
hcfmusp.relation.referenceCaffery B, 2010, MOL VIS, V16, P1720-
hcfmusp.relation.referenceConceicao GMS, 2001, ENVIRON HEALTH PERSP, V109, P347, DOI 10.2307/3434781-
hcfmusp.relation.referenceCorrales RM, 2003, CURR EYE RES, V27, P323, DOI 10.1076/ceyr.27.5.323.17221-
hcfmusp.relation.referenceCorrales RM, 2011, INVEST OPHTH VIS SCI, V52, P8363, DOI 10.1167/iovs.11-7655-
hcfmusp.relation.referencede Toledo Giovana Iara Ferreira Moser, 2011, Rev Bras Epidemiol, V14, P445, DOI 10.1590/S1415-790X2011000300009-
hcfmusp.relation.referenceDogru M, 2005, CORNEA, V24, pS18, DOI 10.1097/01.ico.0000178741.14212.53-
hcfmusp.relation.referenceDoughty MJ, 2012, OCUL SURF, V10, P149, DOI 10.1016/j.jtos.2012.05.001-
hcfmusp.relation.referenceGipson IK, 2011, CORNEA, V30, P1346, DOI 10.1097/ICO.0b013e31820d852a-
hcfmusp.relation.referenceGipson IK, 2004, EXP EYE RES, V78, P379, DOI 10.1016/S0014-4835(03)00204-5-
hcfmusp.relation.referenceGrigg J, 2012, AM J RESP CRIT CARE, V186, P1208, DOI 10.1164/rccm.201210-1892ED-
hcfmusp.relation.referenceGupta SK, 2002, OPHTHALMOLOGICA, V216, P368, DOI 10.1159/000066183-
hcfmusp.relation.referenceJerrett M, 2009, ENVIRON HEALTH PERSP, V117, P772, DOI 10.1289/ehp.11533-
hcfmusp.relation.referenceJOHNSON HG, 1985, AM REV RESPIR DIS, V131, P917-
hcfmusp.relation.referenceKardon R, 1999, INVEST OPHTH VIS SCI, V40, P1328-
hcfmusp.relation.referenceKelly FJ, 2011, RES REP HLTH EFF I, V155, P5-
hcfmusp.relation.referenceKelly FJ, 2012, RESPIROLOGY, V17, P7, DOI 10.1111/j.1440-1843.2011.02065.x-
hcfmusp.relation.referenceKunzli N, 2001, AM J EPIDEMIOL, V153, P1050, DOI 10.1093/aje/153.11.1050-
hcfmusp.relation.referenceMantelli F, 2008, CURR OPIN ALLERGY CL, V8, P477, DOI 10.1097/ACI.0b013e32830e6b04-
hcfmusp.relation.referenceMAROM Z, 1983, J CLIN INVEST, V72, P122, DOI 10.1172/JCI110949-
hcfmusp.relation.referenceMartins LC, 2002, J OCCUP ENVIRON MED, V44, P622, DOI 10.1097/01.jom.0000023250.57933.f2-
hcfmusp.relation.referenceMedina-Ramon M, 2006, AM J EPIDEMIOL, V163, P579, DOI 10.1093/aje/kwj078-
hcfmusp.relation.referenceNovaes P, 2007, ENVIRON HEALTH PERSP, V115, P1753, DOI 10.1289/ehp.10363-
hcfmusp.relation.referenceNovaes P., 2011, ENVIRON RES, V110, P372-
hcfmusp.relation.referencePereira LAA, 1998, ENVIRON HEALTH PERSP, V106, P325, DOI 10.1289/ehp.98106325-
hcfmusp.relation.referencePires-Neto RC, 2006, ENVIRON RES, V101, P356, DOI 10.1016/j.envres.2005.12.018-
hcfmusp.relation.referencePisella PJ, 2000, OPHTHALMOLOGY, V107, P1841, DOI 10.1016/S0161-6420(00)00347-X-
hcfmusp.relation.referencePrigol AM, 2012, ARQ BRAS OFTALMOL, V75, P24, DOI 10.1590/S0004-27492012000100005-
hcfmusp.relation.referenceSALDIVA PHN, 1995, ARCH ENVIRON HEALTH, V50, P159-
hcfmusp.relation.referenceSamoli E, 2003, OCCUP ENVIRON MED, V60, P977, DOI 10.1136/oem.60.12.977-
hcfmusp.relation.referenceSantos UD, 2005, EUR HEART J, V26, P193, DOI 10.1093/eurheartj/ehi035-
hcfmusp.relation.referenceSaxena R, 2003, ACTA OPHTHALMOL SCAN, V81, P491, DOI 10.1034/j.1600-0420.2003.00119.x-
hcfmusp.relation.referenceSchiffman RM, 2000, ARCH OPHTHALMOL-CHIC, V118, P615-
hcfmusp.relation.referenceSingh R, 2005, BRIT J OPHTHALMOL, V89, P1655, DOI 10.1136/bjo.2005.073916-
hcfmusp.relation.referenceSouza MB, 1998, CHEST, V113, P1312, DOI 10.1378/chest.113.5.1312-
hcfmusp.relation.referenceTei M, 2000, INVEST OPHTH VIS SCI, V41, P82-
hcfmusp.relation.referenceTorricelli AAM, 2013, CORNEA, V32, pE11, DOI 10.1097/ICO.0b013e31825e845d-
hcfmusp.relation.referenceTorricelli AAM, 2011, ARQ BRAS OFTALMOL, V74, P377, DOI 10.1590/S0004-27492011000500016-
hcfmusp.relation.referenceVersura P, 1999, OPHTHALMOLOGICA, V213, P103, DOI 10.1159/000027401-
hcfmusp.relation.referenceWolkoff P, 2010, TOXICOL LETT, V199, P203, DOI 10.1016/j.toxlet.2010.09.001-
hcfmusp.relation.referenceYANNI JM, 1989, INT ARCH ALLER A IMM, V90, P307-
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Artigos e Materiais de Revistas Científicas - FM/MOF
Departamento de Otorrinolaringologia e Oftalmologia - FM/MOF

Artigos e Materiais de Revistas Científicas - FM/MPT
Departamento de Patologia - FM/MPT

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - HC/InCor
Instituto do Coração - HC/InCor

Artigos e Materiais de Revistas Científicas - LIM/05
LIM/05 - Laboratório de Poluição Atmosférica Experimental

Artigos e Materiais de Revistas Científicas - LIM/33
LIM/33 - Laboratório de Oftalmologia

Artigos e Materiais de Revistas Científicas - LIM/55
LIM/55 - Laboratório de Urologia

Artigos e Materiais de Revistas Científicas - ODS/03
ODS/03 - Saúde e bem-estar

Artigos e Materiais de Revistas Científicas - ODS/11
ODS/11 - Cidades e comunidades sustentáveis

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