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dc.contributorSistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP-
dc.contributor.authorJANZ, Felipe de Lara-
dc.contributor.authorDEBES, Adriana de Aguiar-
dc.contributor.authorCAVAGLIERI, Rita de Cassia-
dc.contributor.authorDUARTE, Sergio Aloisio-
dc.contributor.authorROMAO, Carolina Martinez-
dc.contributor.authorMORON, Antonio Fernandes-
dc.contributor.authorZUGAIB, Marcelo-
dc.contributor.authorBYDLOWSKI, Sergio Paulo-
dc.identifier.citationJOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, article ID 649353, 10p, 2012-
dc.description.abstractAmniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing. AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages. All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed. In this concern, dimethyl sulfoxide (Me2SO) showed the best results. The freezing protocols tested did not promote significant changes in the AFSCs viability. Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months. Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios.-
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)-
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)-
dc.description.sponsorshipInstituto Nacional de Ciencia e Tecnologia-Fluidos Complexos (INCT-FCx), Brazil-
dc.relation.ispartofJournal of Biomedicine and Biotechnology-
dc.subject.otherdifferentiation protocols-
dc.titleEvaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation-
dc.subject.wosBiotechnology & Applied Microbiology-
dc.subject.wosMedicine, Research & Experimental-
dc.type.categoryoriginal article-
dc.type.versionpublishedVersion-, Adriana de Aguiar:Fundacao Prosangue Hemoctr Sao Paulo, BR-05403000 Sao Paulo, Brazil-, Sergio Aloisio:Univ Sao Paulo, Sch Med, Dept Gynecol & Obstet, BR-05403000 Sao Paulo, Brazil-, Antonio Fernandes:Univ Fed Sao Paulo, Sch Med, Dept Obstet, BR-04021001 Sao Paulo, Brazil-
hcfmusp.publisher.cityNEW YORK-
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hcfmusp.remissive.sponsorshipFederico Foundation-
Appears in Collections:

Artigos e Materiais de Revistas Científicas - FM/MCM
Departamento de Clínica Médica - FM/MCM

Artigos e Materiais de Revistas Científicas - FM/MOG
Departamento de Obstetrícia e Ginecologia - FM/MOG

Artigos e Materiais de Revistas Científicas - HC/ICHC
Instituto Central - HC/ICHC

Artigos e Materiais de Revistas Científicas - LIM/31
LIM/31 - Laboratório de Genética e Hematologia Molecular

Artigos e Materiais de Revistas Científicas - LIM/57
LIM/57 - Laboratório de Fisiologia Obstétrica

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