Por favor, use este identificador para citar o enlazar este ítem: https://observatorio.fm.usp.br/handle/OPI/8466
Título: Immunoproteome of Aspergillus fumigatus Using Sera of Patients with Invasive Aspergillosis
Autor: VIRGINIO, Emylli D.KUBITSCHEK-BARREIRA, Paula H.BATISTA, Marjorie VieiraSCHIRMER, Marcelo R.ABDELHAY, ElianaSHIKANAI-YASUDA, Maria A.LOPES-BEZERRA, Leila M.
Citación: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.15, n.8, p.14505-14530, 2014
Resumen: Invasive aspergillosis is a life-threatening lung or systemic infection caused by the opportunistic mold Aspergillus fumigatus. The disease affects mainly immunocompromised hosts, and patients with hematological malignances or who have been submitted to stem cell transplantation are at high risk. Despite the current use of Platelia (TM) Aspergillus as a diagnostic test, the early diagnosis of invasive aspergillosis remains a major challenge in improving the prognosis of the disease. In this study, we used an immunoproteomic approach to identify proteins that could be putative candidates for the early diagnosis of invasive aspergillosis. Antigenic proteins expressed in the first steps of A. fumigatus germination occurring in a human host were revealed using 2-D Western immunoblots with the serum of patients who had previously been classified as probable and proven for invasive aspergillosis. Forty antigenic proteins were identified using mass spectrometry (MS/MS). A BLAST analysis revealed that two of these proteins showed low homology with proteins of either the human host or etiological agents of other invasive fungal infections. To our knowledge, this is the first report describing specific antigenic proteins of A. fumigatus germlings that are recognized by sera of patients with confirmed invasive aspergillosis who were from two separate hospital units.
Aparece en las colecciones:

Artigos e Materiais de Revistas Científicas - FM/MIP
Departamento de Moléstias Infecciosas e Parasitárias - FM/MIP

Artigos e Materiais de Revistas Científicas - LIM/48
LIM/48 - Laboratório de Imunologia


Ficheros en este ítem:
Fichero Descripción TamañoFormato 
art_VIRGINIO_Immunoproteome_of_Aspergillus_fumigatus_Using_Sera_of_Patients_2014.PDFpublishedVersion (English)1.47 MBAdobe PDFVista previa
Visualizar/Abrir

Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.