CD8+T Lymphocyte Expansion, Proliferation and Activation in Dengue Fever

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dc.contributor Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSP MATOS, Andreia Manso de
ROSA, Daniela Santoro FMUSP-HC
SILVA, Wanessa Cardoso da FMUSP-HC
RODRIGUES, Celia Luiza de Lima
OLIVEIRA, Olimpia Massae Nakasone Peel Furtado
LEVI, Jose Eduardo FMUSP-HC
ARAUJO, Evaldo Stanislau Affonso FMUSP-HC
PANNUTI, Claudio Sergio FMUSP-HC
LUNA, Expedito Jose Albuquerque FMUSP-HC
KALLAS, Esper George FMUSP-HC 2015
dc.identifier.citation PLOS NEGLECTED TROPICAL DISEASES, v.9, n.2, article ID e0003520, 14p, 2015
dc.identifier.issn 1935-2735
dc.description.abstract Dengue fever induces a robust immune response, including massive T cell activation. The level of T cell activation may, however, be associated with more severe disease. In this study, we explored the level of CD8+ T lymphocyte activation in the first six days after onset of symptoms during a DENV2 outbreak in early 2010 on the coast of Sao Paulo State, Brazil. Using flow cytometry we detected a progressive increase in the percentage of CD8+ T cells in 74 dengue fever cases. Peripheral blood mononuclear cells from 30 cases were thawed and evaluated using expanded phenotyping. The expansion of the CD8+ T cells was coupled with increased Ki67 expression. Cell activation was observed later in the course of disease, as determined by the expression of the activation markers CD38 and HLA-DR. This increased CD8+ T lymphocyte activation was observed in all memory subsets, but was more pronounced in the effector memory subset, as defined by higher CD38 expression. Our results show that most CD8+ T cell subsets are expanded during DENV2 infection and that the effector memory subset is the predominantly affected sub population.
dc.description.sponsorship · Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazilian Ministry of Science and Technology (CNPq) [476088/2009-7]
· CNPq
· Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazilian Ministry of Education
dc.language.iso eng
dc.relation.ispartof Plos Neglected Tropical Diseases
dc.rights openAccess
dc.subject.other cd8(+) t-cells; hemorrhagic-fever; viral-infections; virus-infection; memory; subsets; responses; disease; differentiation; pathogenesis
dc.title CD8+T Lymphocyte Expansion, Proliferation and Activation in Dengue Fever
dc.type article
dc.rights.holder Copyright PUBLIC LIBRARY SCIENCE LIM/52 LIM/47 LIM/60
dc.identifier.doi 10.1371/journal.pntd.0003520
dc.identifier.pmid 25675375
dc.type.category original article
dc.type.version publishedVersion CARVALHO, Karina Inacio:FM: ROSA, Daniela Santoro:FM: VILLAS-BOAS, Lucy Santos:HC:LIM/52 SILVA, Wanessa Cardoso da:FM: LEVI, Jose Eduardo:HC:LIM/52 ARAUJO, Evaldo Stanislau Affonso:HC:LIM/47 PANNUTI, Claudio Sergio:FM:MIP LUNA, Expedito Jose Albuquerque:IMT:IMT KALLAS, Esper George:FM:MCM · MATOS, Andreia Manso de:Univ Sao Paulo, Fac Med, Disciplina Imunol Clin & Alergia LIM 60, Sao Paulo, Brazil
· RODRIGUES, Celia Luiza de Lima:Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, Brazil; Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas & Parasitarias LIM 52, Sao Paulo, Brazil
· OLIVEIRA, Olimpia Massae Nakasone Peel Furtado:Hosp Ana Costa, Sao Paulo, Brazil WOS:000350992500048 2-s2.0-84924082739 SAN FRANCISCO USA
hcfmusp.relation.reference · Appay V, 2008, CYTOM PART A, V73A, P975, DOI 10.1002/cyto.a.20643
· Azeredo EL, 2006, MEM I OSWALDO CRUZ, V101, P437, DOI 10.1590/S0074-02762006000400016
· Bashyam HS, 2006, J IMMUNOL, V176, P2817
· Bhatt S, 2013, NATURE, V496, P504, DOI 10.1038/nature12060
· Capeding MR, 2014, LANCET, V384, P1358, DOI 10.1016/S0140-6736(14)61060-6
· Chau Tran Nguyen Bich, 2008, J Infect Dis, V198, P516, DOI 10.1086/590117
· CORLEY RB, 1985, P NATL ACAD SCI USA, V82, P516, DOI 10.1073/pnas.82.2.516
· de Souza VAUF, 2004, J CLIN MICROBIOL, V42, P1782, DOI 10.1128/JCM.42.4.1782-1784.2004
· Dung Nguyen Thi Phuong, 2010, J Immunol, V184, P7281, DOI 10.4049/jimmunol.0903262
· Durbin AP, 2008, VIROLOGY, V376, P429, DOI 10.1016/j.virol.2008.03.028
· Endy TP, 2004, J INFECT DIS, V189, P990, DOI 10.1086/382280
· Friberg H, 2011, IMMUNOL CELL BIOL, V89, P122, DOI 10.1038/icb.2010.61
· Geginat J, 2003, BLOOD, V101, P4260, DOI 10.1182/blood-2002-11-3577
· Goncalves RM, 2010, INFECT IMMUN, V78, P4763, DOI 10.1128/IAI.00578-10
· Green S, 1999, J INFECT DIS, V180, P1429, DOI 10.1086/315072
· Guidotti LG, 2001, ANNU REV IMMUNOL, V19, P65, DOI 10.1146/annurev.immunol.19.1.65
· Guirakhoo F, 2001, J VIROL, V75, P7290, DOI 10.1128/JVI.75.16.7290-7304.2001
· Halstead SB, 2007, LANCET, V370, P1644, DOI 10.1016/S0140-6736(07)61687-0
· HALSTEAD SB, 1988, SCIENCE, V239, P476, DOI 10.1126/science.3277268
· Lai YL, 2007, J CLIN MICROBIOL, V45, P935, DOI 10.1128/JCM.01258-06
· Lanzavecchia A, 2005, CURR OPIN IMMUNOL, V17, P326, DOI 10.1016/j.coi.2005.04.010
· Levi JE, 2007, J CLIN MICROBIOL, V45, P1893, DOI 10.1128/JCM.00065-07
· Liu CC, 2002, J MED VIROL, V68, P241, DOI 10.1002/jmv.10198
· Malavige GN, 2004, POSTGRAD MED J, V80, P588, DOI 10.1136/pgmj.2004.019638
· Mathew A, 1999, J IMMUNOL, V162, P5609
· Mathew A, 2008, IMMUNOL REV, V225, P300, DOI 10.1111/j.1600-065X.2008.00678.x
· McMichael AJ, 2000, PHILOS T ROY SOC B, V355, P363
· Messer WB, 2002, AM J TROP MED HYG, V66, P765
· Roederer M, 2001, CYTOMETRY, V45, P194, DOI 10.1002/1097-0320(20011101)45:3<194::AID-CYTO1163>3.0.CO;2-C
· Romano CM, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0011811
· Sallusto F, 1999, NATURE, V401, P708, DOI 10.1038/44385
· SARASOMBATH S, 1988, Southeast Asian Journal of Tropical Medicine and Public Health, V19, P649
· Schmid ML, 1999, BRIT MED J, V318, P1076
· Tomiyama H, 2002, J IMMUNOL, V168, P5538
· Villar L, 2014, NEW ENGL J MED, DOI [10. 1056/ NEJMsr1414226, DOI 10.1056/NEJMSR1414226]
· Weiskopf D, 2015, J VIROL, V89, P120, DOI 10.1128/JVI.02129-14
· Weiskopf D, 2013, P NATL ACAD SCI USA, V110, pE2046, DOI 10.1073/pnas.1305227110
· Zielinski CE, 2011, IMMUNOL REV, V240, P40, DOI 10.1111/j.1600-065X.2010.01000.x
dc.description.index MEDLINE
hcfmusp.citation.scopus 18 Brasil
hcfmusp.scopus.lastupdate 2021-06-22

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