Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPSILVA, Luan CesarLEITE, Amanda AlmeidaBORGATO, Gabriell BonifacioWAGNER, Vivian PetersenMARTINS, Manoela DominguesLOUREIRO, Felippe Jose AlmeidaLOPES, Marcio AjudarteSANTOS-SILVA, Alan RogerSPERANDIO, MarceloJUNIOR, Gilberto de CastroKOWALSKI, Luiz PauloSQUARIZE, Cristiane H.CASTILHO, Rogerio MoraesVARGAS, Pablo Agustin2024-02-152024-02-152023AMERICAN JOURNAL OF CANCER RESEARCH, v.13, n.12, p.6038-6050, 20232156-6976https://observatorio.fm.usp.br/handle/OPI/57914Despite many progresses in the development of new systemic therapies for oral squamous cell carcinoma (OSCC), the five-year survival rate of OSCC is low. The traditional chemotherapies approach (cisplatin - CDDP) shows some limitations like drug toxicity, limited efficacy, and drug resistance. Promising studies suggested OSCC cancer stem cells (CSC) presented resistance to CDDP. We have previously studied many targets, and we extensively showed the efficacy of the NF kappa B signaling and the role of histones acetylation, on different malignant tumors, including adenoid cystic carcinoma and mucoepidermoid carcinoma, but until then the effects of the NFkB inhibitor and histone deacetylase (HDAC) inhibitor on the biology of OSCC were not evaluated. Here we assessed the pharmacological inhibitor of NF kappa B emetine and HDAC inhibitor SAHA on the behavior of CSC derived from OSCC. Our data suggested that CDDP administration resulted in reduced viability of bulk OSCC cells and increased CSC. A single and isolated shot of emetine and SAHA were able to disrupt CSC by inhibiting the NF kappa B pathway and increasing the histone acetylation levels, respectively. Further, the combined administration of emetine and SAHA presented the same CSC disruption as seen in emetine alone.engrestrictedAccessHead and neck tumorsoral squamous cell carcinomachemotherapytarget therapycancer stem cellheadexpressioncisplatintherapyemetinearticleCopyright E-CENTURY PUBLISHING CORPOncology