Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPSIQUEIRA, Lumena P. MachadoGIMENES, Viviane M. FaveroFREITAS, Roseli Santos deMELHEM, Marcia de Souza CarvalhoBONFIETTI, Lucas XavierJR, Afonso Rafael da SilvaSANTOS, Leticia B. SouzaMOTTA, Adriana L.ROSSI, FlaviaBENARD, GilJR, Joao N. de Almeida2019-02-212019-02-212019JOURNAL OF CLINICAL MICROBIOLOGY, v.57, n.1, article ID UNSP e01282, 7p, 20190095-1137https://observatorio.fm.usp.br/handle/OPI/30836Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proposed as an alternative method for labor-intensive methods for C. neoformans and C. gattii genotype differentiation. However, Vitek MS, one of the commercial MALDI-TOF MS instruments, has not been yet been evaluated for this purpose. Thus, we constructed an in-house database with reference strains belonging to the different C. neoformans (VNI, VNII, VNIII, and VNIV) and C. gattii (VGI, VGII, VGIII, and VGIV) major molecular types by using the software Saramis Premium (bioMerieux, Marcy-l'Etoile, France). Then, this new database was evaluated for discrimination of the different genotypes. Our in-house database provided correct identification for all C. neoformans and C. gattii genotypes; however, due to the intergenotypic mass spectral similarities, a careful postanalytic evaluation is necessary to provide correct genotype identification.engrestrictedAccessCryptococcus gattiiCryptococcus neoformansMALDI-TOFVITEK MSgenotypic identificationdesorption ionization-timeflight mass-spectrometrymolecular typesidentificationcomplexivdarticleCopyright AMER SOC MICROBIOLOGY10.1128/JCM.01282-18Microbiology1098-660X