Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPSALOMONE, RaquelCOSTA, Heloisa Juliana Zabeu RossiRODRIGUES, Jose Ricardo FerreiraSILVA, Samanta Marques Reis eORANDO, Patricia CamachoBENTO, Ricardo Ferreira2013-07-302013-07-302012ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, v.121, n.3, p.179-184, 20120003-4894https://observatorio.fm.usp.br/handle/OPI/902Objectives: Our objective was to develop an experimental model for the noninvasive and objective evaluation of facial nerve regeneration in rats using a motor nerve conduction test (electromyography). Methods: Twenty-two rats were submitted to neurophysiological evaluation using motor nerve conduction of the mandibular branch of the facial nerve to obtain the compound muscle action potentials (CMAPs). To record the CM APs, we used two needle electrodes that were inserted into the lower lip muscle of the rat. A supramaximal electrical stimulus was applied, and the values of CMAP latency, amplitude, length, area, and stimulus intensity obtained from each side were compared by use of the Wilcoxon test. Results: There was no significant difference (all p > 0.05) in latency, amplitude, duration, area, or intensity of stimuli between the two sides. The amplitudes ranged between 1.61 and 8.30 mV, the latencies between 1.03 and 1.97 ms, and the stimulus intensities between 1.50 and 2.90 mA. Conclusions: This is a noninvasive, easy, and highly reproducible method that contributes to an improvement of the techniques previously described and may contribute to future studies of the degeneration and regeneration of the facial nerve.engrestrictedAccesselectromyographyfacial nervenerve regenerationneurophysiologyWistar ratregenerationmotoneuronstransectioncompressionrecoveryinjurysuturemousearticleCopyright ANNALS PUBL COOtorhinolaryngology