Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPYAEGASHI, Lygia BertalhaBALDAVIRA, Camila MachadoPRIETO, Tabatha GutierrezMACHADO-RUGOLO, JulianaVELOSA, Ana Paula PereiraSILVEIRA, Lizandre Keren Ramos daASSATO, AlineAB'SABER, Alexandre MuxfeldtFALZONI, RobertoTAKAGAKI, TeresaSILVA, Pedro LemeTEODORO, Walcy RosoliaCAPELOZZI, Vera Luiza2021-10-202021-10-202021FRONTIERS IN IMMUNOLOGY, v.12, 20211664-3224https://observatorio.fm.usp.br/handle/OPI/42384Non-small cell lung carcinoma (NSCLC) is a complex cancer biome composed of malignant cells embedded in a sophisticated tumor microenvironment (TME) combined with different initiating cell types, including immune cells and cancer-associated fibroblasts (CAFs), and extracellular matrix (ECM) proteins. However, little is known about these tumors' immune-matricellular relationship as functional and mechanical barriers. This study investigated 120 patients with NSCLC to describe the immune-matricellular phenotypes of their TME and their relationship with malignant cells. Immunohistochemistry (IHC) was performed to characterize immune checkpoints (PD-L1, LAG-3, CTLA-4+, VISTA 1), T cells (CD3+), cytotoxic T cells (CD8(+), Granzyme B), macrophages (CD68+), regulatory T cells (FOXP3+, CD4+), natural killer cells (CD57+), and B lymphocytes (CD20+), whereas CAFs and collagen types I, III, and V were characterized by immunofluorescence (IF). We observed two distinct functional immune-cellular barriers-the first of which showed proximity between malignant cells and cytotoxic T cells, and the second of which showed distant proximity between non-cohesive nests of malignant cells and regulatory T cells. We also identified three tumor-associated matricellular barriers: the first, with a localized increase in CAFs and a low deposition of Col V, the second with increased CAFs, Col III and Col I fibers, and the third with a high amount of Col fibers and CAFs bundled and aligned perpendicularly to the tumor border. The Cox regression analysis was designed in two steps. First, we investigated the relationship between the immune-matricellular components and tumor pathological stage (I, II, and IIIA), and better survival rates were seen in patients whose tumors expressed collagen type III > 24.89 fibers/mm(2). Then, we included patients who had progressed to pathological stage IV and found an association between poor survival and tumor VISTA 1 expression > 52.86 cells/mm(2) and CD3+ <= 278.5 cells/mm(2). We thus concluded that differential patterns in the distribution of immune-matricellular phenotypes in the TME of NSCLC patients could be used in translational studies to predict new treatment strategies and improve patient outcome. These data raise the possibility that proteins with mechanical barrier function in NSCLC may be used by cancer cells to protect them from immune cell infiltration and immune-mediated destruction, which can otherwise be targeted effectively with immunotherapy or collagen therapy.engopenAccesslung cancerimmune cellscollagencancer-associated fibroblastsimmunofluorescenceimmunohistochemistrynon-small cell lung cancertumor-infiltrating lymphocytesregulatory t-cellsfoxp3 expressionclinical-significancealigned collagenmigrationmicroenvironmentsurvivalclassificationmacrophagesarticleCopyright FRONTIERS MEDIA SA10.3389/fimmu.2021.714230Immunology