Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPCORTELLA, Lucas R. X.CESTARI, I. A.S, M.MAZZETTO, M.LASAGNI, A. F.CESTARI, Ismar N.2022-11-252022-11-252022XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020, p.119-126, 2022978-3-030-70601-2; 978-3-030-70600-51680-0737https://observatorio.fm.usp.br/handle/OPI/50344The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) to replace myocardial tissue after an infarct holds great promises. However, hiPSC-CM are phenotypically immature when compared to cells in the adult heart, hampering their clinical application. We aimed to develop and test a surface structuring technique that would improve hiPSC-CM structural maturation. Laser ablation was used to fabricate a micron-pattern on polyurethane surface and evaluated cell morphology, orientation and F-actin assemblage to detect phenotypic changes in response to the microtopography. This topography positively influenced cell morphology regarding to spreading area and elongation, and hiPSC-CM orientation, improving their structural maturation. The methodology thus presented has relatively low cost and is easily scalable, making it relevant for high-throughput applications such as drug screening for the pharma industry.engrestrictedAccesshiPSC-CMCardiomyocytesSurface topographyDirect laser interference patterningPolyurethanestem-cellconferenceObjectCopyright SPRINGER10.1007/978-3-030-70601-2_20Engineering, Biomedical