Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPSERIANI, RobsonJUNQUEIRA, Mara S.TOLEDO, Alessandra C.CORREA, Aristides T.SILVA, Luiz F. F.MARTINS, Milton A.SALDIVA, Paulo H. N.MAUAD, ThaisMACCHIONE, Mariangela2015-07-012015-07-012015JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v.78, n.4, p.215-225, 20151528-7394https://observatorio.fm.usp.br/handle/OPI/9250Diesel exhaust particles (DEP) contain organic and inorganic elements that produce damage to the respiratory epithelium. The aim of this study was to determine the mucus profile of tracheal explants exposed to either crude diesel exhaust particles (DEP) or DEP treated with nitric acid (DEP/NA), with hexane (DEP/HEX), or with methanol (DEP/MET) at concentrations of 50 and 100 mu g/ml for 30 and 60 min. Tracheal explants were subjected to morphometric analyses to study acidic (AB+), neutral (PAS+), and mixed (AB+/PAS+) mucus production and vacuolization (V). Incubation with 50 mu g/ml crude DEP resulted in a rise in acid mucus production, an increase in vacuolization at 30 min, and reduction in neutral mucus at 30 and 60 min. Tracheas exposed to DEP/MET at 50 mu g/ml for 30 or 60 min resulted in a significant decrease in neutral mucus production and an elevation in acid mucus production. DEP/HEX increased vacuolization at both 50 and 100 mu g/ml at 30 and 60 min of exposure. Treatment with 50 mu g/ml for 30 or 60 min significantly elevated mixed mucus levels. These results suggest that DEP appear to be more toxic when administered in combination with HEX or MET. DEP/MET modified the mucus profile of the epithelium, while DEP/HEX altered mucus extrusion, and these responses might be due to bioavailability of individual elements in DEP fractions.engrestrictedAccessallergic airway inflammationbronchial epithelial-cellspulmonary inflammationinhalation exposurepollution particlesoxidative stresssulfur-dioxideterm exposurenasal-mucosamicearticleCopyright TAYLOR & FRANCIS INC10.1080/15287394.2014.947456Environmental SciencesPublic, Environmental & Occupational HealthToxicology1087-2620