Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPFERREIRA, Leandro RodriguesKESPER, NorivalTEIXEIRA, Marta M. G.LAURENTI, Marcia DalastraBARBIERI, C. L.LINDOSO, Jose AngeloUMEZAWA, Eufrosina S.2014-09-302014-09-302014ACTA TROPICA, v.131, p.41-46, 20140001-706Xhttps://observatorio.fm.usp.br/handle/OPI/7700We investigated whether ELISA using crude antigens from insect and plant trypanosomatids, which are non-pathogenic and easily cultivated in large scale, has the same positivity data as Leishmania (Leishmania) chagasi, the etiological agent of human visceral leishmaniasis (VL) or canine leishmaniasis (CanL), or as Trypanosoma cruzi, the etiological agent of Chagas disease (CD). The antigens from Crithidia fasciculata, Crithidia luciliae, and Leptomonas seymouri showed 100% cross-reactivity with VL and CanL samples, with no statistically titers differences from L. (L.) chagasi, however, 34% (17/50) of VL samples revealed higher titers using the insect trypanosomatids than the homologous antigen. On the other hand, antigens from Strigomonas culicis, Angomonas deanei, and Phytomonas serpens showed low cross-reactivity with VL and CanL samples. The sera from patients with American tegumentary leishmaniasis showed low levels of cross-reactivity with all trypanosomatids investigated, even with L. (L) chagasi, without titers dissimilarity among them. These parasites were also worthless as antigen source for detection of CD cases, which required homologous antigens to reach 100% positivity. This study showed, by ELISA, that crude extract of Crithidia and Leptomonas have epitopes similar to L. (L.) chagasi, which supports the idea of using them as antigens source for the serodiagnosis of visceral leishmaniasis.engrestrictedAccessL. (L.) chagasiTrypanosoma cruziCrithidiaLeptomonasTrypanosomatidsCross-reactivityexcreted-secreted antigensphytomonas-serpensherpetomonas-samuelpessoaiinsect trypanosomatidsvisceral leishmaniasisprotective immunitytomato parasitecruzidiagnosisimmunofluorescencearticleCopyright ELSEVIER SCIENCE BV10.1016/j.actatropica.2013.11.010ParasitologyTropical Medicine1873-6254