Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPGRYSCHEK, Ronaldo Cesar BorgesCORRAL, Marcelo AndreettaSITTA, Renata BarnabeGOTTARDI, MaiaraPIERROTTI, Ligia CameraCOSTA, Silvia FigueiredoABDALA, EdsonCHIEFFI, Pedro PauloPAULA, Fabiana Martins de2024-02-152024-02-152023TRANSPLANT INFECTIOUS DISEASE, v.25, n.6, 20231398-2273https://observatorio.fm.usp.br/handle/OPI/57945Background: The potential that Strongyloides stercoralis infection has to cause major morbidity and high mortality when the disseminated form occurs in transplant patients is of particular concern.Methods: In this study, the objective was to observe S. stercoralis infection in patients who are candidates for transplantation by using parasitological, serological, and molecular techniques and to propose an algorithm for the detection of that infection in transplant candidates.Results: By parasitological techniques, 10% of fecal samples were positive. Anti-Strongyloides antibodies immunoglobulin G were detected in 19.3% and 20.7% of patients by immunofluorescence assay and enzyme-linked immunosorbent assay, respectively. S. stercoralis DNA was observed in 17.3% of samples by conventional polymerase chain reaction and 32.7% of samples by quantitative polymerase chain reaction (qPCR).Conclusion: The set of results allows us to reinforce that a positive result by parasitological techniques and/or qPCR indicates that the specific treatment should be applied. However, the improvement of diagnostic techniques may suggest changes in the screening for strongyloidiasis in these patients. imageengrestrictedAccessdiagnosis methodsStrongyloides infectiontransplant candidatesreal-time pcrmolecular diagnosishyperinfection syndromedrug-resistanceendemic areastercoralisvenezuelensissamplesimmunodiagnosisantigensarticleCopyright WILEY10.1111/tid.14153ImmunologyInfectious DiseasesTransplantation1399-3062