Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPBOTELHO, Patricia BorgesGUIMARAES, Jessica PereiraMARIANO, Karina RochaAFONSO, Milessa da SilvaKOIKE, Marcia KiyomiLOTTENBERG, Ana Maria PitaCASTRO, Inar Alves2015-12-102015-12-102015EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, v.117, n.10, p.1561-1568, 20151438-7697https://observatorio.fm.usp.br/handle/OPI/12534Bioactive compounds may be an alternative approach to prevent atherosclerosis. To evaluate this hypothesis, LDLr-knockout mice were supplemented with omega-3 fatty acids from Echium oil (10.24mg/d of oil with 1,14mg/d of SDA and 9.06mg/d of ALA) equivalent to 0.7mg/d of EPA after conversion, combined or not with phytosterols (0.76mg/d), during the first 2 months of life. Subsequently, dyslipidaemia was induced by a high-fat diet for the following 2 months. Echium oil, isolated or combined with phytosterols, improved lipid profile in plasma reducing triacylglycerol (90.37.6mg/dL) and VLDL-c (18.01.5mg/dL) concentrations when compared with Control (115.8 +/- 9.4mg/dL and 23.2 +/- 1.9mg/dL, respectively). Echium oil also increased catalase (5.66 +/- 0.13U/mg protein) while Echium oil combined with phytosterol increased glutathione peroxidase activity (26.27 +/- 0.10U/mg protein) when compared with Control (5.18 +/- 0.10U/mg protein and 25.31 +/- 0.16U/mg protein, respectively). In addition, groups receiving Echium oil have reduced malondialdehyde concentration in liver (p=0.05). However, no difference was observed in fatty streak lesions when compared with Control. Isolated phytosterols did not change cholesterol absorption and increased lesion area compared with control group. This result can be associated with the high dose applied in the first step of supplementation and with the form of supplementation (gavage). Practical applications: One factor that contributes to the number of deaths from cardiovascular disease is that pharmacological interventions usually start too late in life. For this reason, functional foods development is a very important strategy to prevent atherosclerosis, since their inclusion in diet can start much earlier. However, it represents a challenge because many physiological responses from chronic consumption of bioactive compounds are still unknown. In this study, considering the positive results on triglyceridemia and oxidative stress biomarkers, we suggest that Echium oil can be an alternative for development of functional foods. We selected Echium oil due to its higher proportion of stearidonic fatty acids (pro-EPA) and lower sensory limitation than marine oils. Echium oil improves lipid profile and reduces oxidative stress, while phytosterol increases fatty streak.engrestrictedAccessAtherosclerosisEchiumMiceOmega 3Oxidative stressPhytosterolscholesterol-metabolismplant sterolsplasmaomega-3-fatty-acidsmechanismsdiseasestanolslesionslivergenearticleCopyright WILEY-BLACKWELL10.1002/ejlt.201500004Food Science & TechnologyNutrition & Dietetics1438-9312