Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPFERREIRA, Noely EvangelistaCOSTA, Antonio C. daKALLAS, Esper G.SILVEIRA, Cassia G. T.OLIVEIRA, Ana Carolina S. deHONORATO, LaylaPAIAO, Heuder G. O.LIMA, Silvia H.VASCONCELOS, Dewton de M.CORTES, Marina F.COSTA, Silvia F.MENDOZA, Tania R. T.GOMES, Helio R.WITKIN, Steven S.MENDES-CORREA, Maria C.2024-02-152024-02-152023CURRENT ISSUES IN MOLECULAR BIOLOGY, v.45, n.9, p.6958-6966, 20231467-3037https://observatorio.fm.usp.br/handle/OPI/57950Parvovirus B19 infection is associated with a wide range of clinical manifestations, from asymptomatic to severe neurological disorders. Its major clinical symptoms, fever and rash, are common to multiple viruses, and laboratory tests to detect B19 are frequently not available. Thus, the impact of B19 on public health remains unclear. We report the case of a 38-day old girl admitted to Sao Paulo Clinical Hospital, Brazil, with an initial diagnosis of bacterial meningitis, seizures, and acute hydrocephalus. Antibiotic therapy was maintained for one week after admission and discontinued after negative laboratory results were obtained. Nine days after symptoms onset, a cerebral spinal fluid (CSF) sample revealed persistent pleocytosis. The complete B19 complete genome was subsequently identified in her CSF by a metagenomic next-generation sequencing approach. This report highlights the possible involvement of B19 in the occurrence of acute neurological manifestations and emphasizes that its possible involvement might be better revealed by the use of metagenomic technology to detect viral agents in clinical situations of unknown or uncertain etiology.engopenAccesshuman parvovirus B19encephalopathymetagenomic next-generation sequencingcerebrospinal-fluidinfectionmeningoencephalitisvirusencephalitisactivationgenernaarticleCopyright MDPI10.3390/cimb45090439Biochemistry & Molecular Biology1467-3045