Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPCOSTA, Antonio Charlys daLUCHS, AdrianaMILAGRES, Flavio Augusto de PaduaKOMNINAKIS, Shirley VasconcelosGILL, Danielle EliseLOBATO, Marcia Cristina Alves Brito SayaoBRUSTULIN, RafaelCHAGAS, Rogerio Togisaki dasABRAO, Maria de Fatima Neves dos SantosSOARES, Cassia Vitoria de Deus AlvesDENG, XutaoSABINO, Ester CerdeiraDELWART, EricLEAL, Elcio2019-01-172019-01-172018VIRUSES-BASEL, v.10, n.10, article ID 520, 12p, 20181999-4915https://observatorio.fm.usp.br/handle/OPI/29949Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes.engopenAccesssaffold viruscardiovirusviromepicornavirusribosomal frameshiftingGGUUUUU motifRNA-dependent RNA-polymeraseinfectionschildrenvirusesarticleCopyright MDPI10.3390/v10100520Virology