Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPLEVI, Jose EduardoNASCIMENTO, Maria ClaudiaSUMITA, Laura MasamiSOUZA, Vanda Akico Ueda Fick deFREIRE, Wilton S.MAYAUD, PhilippePANNUTI, Claudio S.2017-11-272017-11-272011PLOS ONE, v.6, n.8, article ID e23546, 4p, 20111932-6203https://observatorio.fm.usp.br/handle/OPI/23052Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), is the etiologic agent of all forms of Kaposi's sarcoma, primary effusion lymphoma and the plasmablastic cell variant of multicentric Castleman disease. In endemic areas of sub-Saharan Africa, blood transfusions have been associated with a substantial risk of HHV-8 transmission. By contrast, several studies among healthy blood donors from North America have failed to detect HHV-8 DNA in samples of seropositive individuals. In this study, using a real-time PCR assay, we investigated the presence of HHV-8 DNA in whole-blood samples of 803 HHV-8 blood donors from three Brazilian states (Sao Paulo, Amazon, Bahia) who tested positive for HHV-8 antibodies, in a previous multicenter study. HHV-8 DNA was not detected in any sample. Our findings do not support the introduction of routine HHV-8 screening among healthy blood donors in Brazil. (WC = 140).engopenAccesshuman-herpesvirus type-8latent class analysiskaposis-sarcomamononuclear-cellsrisk-factorsviral loadtransmissiontransfusionprevalenceinfectionarticleCopyright PUBLIC LIBRARY SCIENCE10.1371/journal.pone.0023546Multidisciplinary Sciences