Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPMORAES, Wilson Max Almeida Monteiro DeSOUZA, Pamella Ramona Moraes dePAIXAO, Nathalie Alves daSOUSA, Luis Gustavo Oliveira deRIBEIRO, Daniel ArakiMARSHALL, Andrea G.PRESTES, JonatoIRIGOYEN, Maria ClaudiaBRUM, Patricia ChakurMEDEIROS, Alessandra2018-05-082018-05-082018JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, v.22, n.3, p.1452-1463, 20181582-4934https://observatorio.fm.usp.br/handle/OPI/26526We tested whether aerobic exercise training (AET) would modulate the skeletal muscle protein quality control (PQC) in a model of chronic kidney disease (CKD) in rats. Adult Wistar rats were evaluated in four groups: control (CS) or trained (CE), and 5/6 nephrectomy sedentary (5/6NxS) or trained (5/6NxE). Exercised rats were submitted to treadmill exercise (60min., five times/wk for 2months). We evaluated motor performance (tolerance to exercise on the treadmill and rotarod), cross-sectional area (CSA), gene and protein levels related to the unfolded protein response (UPR), protein synthesis/survive and apoptosis signalling, accumulated misfolded proteins, chymotrypsin-like proteasome activity (UPS activity), redox balance and heat-shock protein (HSP) levels in the tibialis anterior. 5/6NxS presented a trend towards to atrophy, with a reduction in motor performance, down-regulation of protein synthesis and up-regulation of apoptosis signalling; increases in UPS activity, misfolded proteins, GRP78, derlin, HSP27 and HSP70 protein levels, ATF4 and GRP78 genes; and increase in oxidative damage compared to CS group. In 5/6NxE, we observed a restoration in exercise tolerance, accumulated misfolded proteins, UPS activity, protein synthesis/apoptosis signalling, derlin, HSPs protein levels as well as increase in ATF4, GRP78 genes and ATF6 protein levels accompanied by a decrease in oxidative damage and increased catalase and glutathione peroxidase activities. The results suggest a disruption of PQC in white muscle fibres of CKD rats previous to the atrophy. AET can rescue this disruption for the UPR, prevent accumulated misfolded proteins and reduce oxidative damage, HSPs protein levels and exercise tolerance.engopenAccesschronic kidney diseaseaerobic exercise trainingskeletal muscleprotein quality controlunfolded protein responsechronic-renal-failureubiquitin-proteasome pathwayendoplasmic-reticulum stressoxidative stresscatabolic conditionsendurancesurvivaldegradationproteolysisactivationarticleCopyright WILEY10.1111/jcmm.13374Cell BiologyMedicine, Research & Experimental