Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPPAULA, Fabiana M.MALTA, Fernanda M.MARQUES, Priscilla D.MELO, Gessica B.CORRAL, Marcelo A.GOTTARDI, MaiaraPINHO, Joao R. R.GONCALVES, Elenice M. N.CASTILHO, Vera L. P.PIERROTTI, Ligia C.ABDALA, EdsonCOSTA, Silvia F.CHIEFFI, Pedro P.GRYSCHEK, Ronaldo C. B.2018-09-132018-09-132018TRANSPLANT INFECTIOUS DISEASE, v.20, n.4, article ID e12909, 4p, 20181398-2273https://observatorio.fm.usp.br/handle/OPI/28129Strongyloidiasis can occur without any symptoms or as a potentially fatal hyperinfection or disseminated infection, principally in immunosuppressed patients. Our study aimed to evaluate the application of conventional polymerase chain reaction (cPCR) and real-time PCR (qPCR). Polymerase chain reaction (PCR) and real-time PCR (qPCR) targeting the 18S rRNA gene for detection of Strongyloides stercoralis infection among transplant candidates were applied in stool samples obtained from 150 transplant candidates, preliminarily analyzed by parasitological methods. S.stercoralis larvae were visualized in 15/150 (10.0%) transplant candidates by parasitological methods. DNA from S.stercoralis was amplified in 26/150 (17.3%) and 49/150 (32.7%) stool samples of transplant candidates, using cPCR and qPCR, respectively. The results suggest that molecular methods, especially qPCR, should be used as an additional tool for diagnostic of S.stercoralis infection among transplant candidates.engrestrictedAccessmolecular diagnosisStrongyloides stercoralistransplant candidatereal-time pcrhuman stool samplesinfectionhookwormarticleCopyright WILEY10.1111/tid.12909ImmunologyInfectious DiseasesTransplantation1399-3062