Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPMACEDO, Mariana Petaccia deNASCIMENTO, Ellen Caroline ToledoSOARES, Fernando AugustoSANTINI, Fernando CostaCOSTA, Felipe D'AlmeidaCUNHA, Isabela Werneck daMUNHOZ, Rodrigo RamellaMARCHI, Pedro DeJORGE, Thiago William CarnierLEITE, Katia Ramos Moreira2023-10-302023-10-302023CLINICAL PATHOLOGY, v.16, article ID 2632010X231197080, 13p, 20232632-010Xhttps://observatorio.fm.usp.br/handle/OPI/55936Oncogenic neurotrophic tropomyosin receptor kinase gene fusions occur in less than 1% of common cancers. These mutations have emerged as new biomarkers in cancer genomic profiling with the approval of selective drugs against tropomyosin receptor kinase fusion proteins. Nevertheless, the optimal pathways and diagnostic platforms for this biomarker's screening and genomic profiling have not been defined and remain a subject of debate. A panel of national experts in molecular cancer diagnosis and treatment was convened by videoconference and suggested topics to be addressed in the literature review. The authors proposed a testing algorithm for oncogenic neurotrophic tropomyosin receptor kinase gene fusion screening and diagnosis for the Brazilian health system. This review aims to discuss the latest literature evidence and international consensus on neurotrophic tropomyosin receptor kinase gene fusion diagnosis to devise clinical guidelines for testing this biomarker. We propose an algorithm in which testing for this biomarker should be requested to diagnose advanced metastatic tumors without known driver mutations. In this strategy, Immunohistochemistry should be used as a screening test followed by confirmatory next-generation sequencing in immunohistochemistry-positive cases.engopenAccessPrecision medicinemolecular targeted therapybiomarkershigh-throughput nucleotide sequencingpan-trk immunohistochemistrycanadian consensussalivary-glandcancersensitivityoncologylungarticleCopyright SAGE PUBLICATIONS LTD10.1177/2632010X231197080Pathology