Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPSILVESTRE, Marilene ChavesREIS, Vitor Manoel Silva dos2019-01-172019-01-172018ANAIS BRASILEIROS DE DERMATOLOGIA, v.93, n.6, p.829-835, 20180365-0596https://observatorio.fm.usp.br/handle/OPI/29902BACKGROUND: Allergic contact dermatitis to ion nickel (Ni+2) is an inflammatory dermatosis, common in industrialized countries. It involves the activation of nickel-specific T-cells, followed by proliferation and induction of a mixed profile of both proinflammatory and regulatory cytokines, suggesting that several T-cell subtypes (helper - Th and cytotoxic - Tc) are involved. A broader understanding of the cytokine profile may lead to new therapeutic approaches. OBJECTIVES: This study aimed to analyze the cytokines TNF-alpha, INIF-gamma, IL-2, IL-4, IL-10, IL-13, IL-17 and IL-23 using the inununohistochemistry technique in order to try to identify their prevalence in chronic and acute eczema of patients with allergic contact dermatitis to Ni+2. METHODS: We performed an immunohistochemical study for eight cytokines in 20 patients with Ni+2 allergic contact dermatitis, biopsied at the site of chronic eczema, triggered by the patient's daily contact with Ni+2, and at the site of acute eczema caused by nickel sulfate, 48 hours after applying the contact test. RESULTS: The stained samples showed positive results for the eight cytokines studied. TNF-alpha, IFN-gamma, IL-4, IL-13 and IL-17 had a higher prevalence in chronic eczema, IL-2 and IL-23 in acute eczema, and IL-10 presented a similar prevalence in both acute and chronic eczema. However, these prevalences were statistically significant only for IL-4 and IL-13. STUDY LIMITATIONS: Small sample size. CONCLUSIONS: In chronic and acute eczema, we observed the presence of a mixed cytokine profile of the T cell subtypes (Th/Tc), suggesting that the responses are expressed at the same time.engopenAccessAllergy and immunologyCytokinesDermatitisallergic contactDermatitiscontactImmunohistochemistryNickelblood mononuclear-cellsimmune eventsinterleukin-17activationhypersensitivityindividualsimmunologyexpressionsecretiongeneticsarticleCopyright SOC BRASILEIRA DERMATOLOGIA10.1590/abd1806-4841.20187126Dermatology1806-4841