Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPJACOB, Cristina Miuki AbePASTORINO, Antonio CarlosOKAY, Thelma SuelyCASTRO, Ana Paula B. M.GUSHKEN, Andrea Keiko F.WATANABE, Leticia AkiFRUCCHI, Vanessa C. Z.OLIVEIRA, Lea Campos de2014-01-282014-01-282013CLINICS, v.68, n.7, p.1004-1009, 20131807-5932https://observatorio.fm.usp.br/handle/OPI/4174OBJECTIVES: The aim of this cross-sectional study was to evaluate whether interleukin 10 (IL10) and transforming growth factor beta 1 (TGF beta 1) gene polymorphisms were associated with persistent IgE-mediated cow's milk allergy in 50 Brazilian children. The diagnostic criteria were anaphylaxis triggered by cow's milk or a positive double-blind, placebo-controlled food challenge. Tolerance was defined as the absence of a clinical response to a double-blind, placebo-controlled food challenge or cow's milk exposure. METHOD: The genomic DNA of the 50 patients and 224 healthy controls (HCs) was used to investigate five IL10 gene polymorphisms (-3575A/T, -2849A/G, -2763A/C, -1082G/A, -592C/A) and one TGF beta 1 polymorphism (-509C/T). RESULTS: Among the five IL10 polymorphisms analyzed, homozygosis for the G allele at the -1082 position was significantly higher in the patients compared with the healthy controls (p = 0.027) and in the persistent cow's milk allergy group compared with the healthy controls (p = 0.001). CONCLUSIONS: Homozygosis for the G allele at the IL10 -1082G/A polymorphism is associated with the persistent form of cow's milk allergy.engopenAccessFood HypersensitivityCow's Milk AllergyClinical OutcomeGene PolymorphismInterleukin 10Transforming Growth Factor beta 1single nucleotide polymorphismsfood allergypromoter polymorphismsatopic-dermatitist-cellschildrenasthmaassociationil-10populationarticleCopyright HOSPITAL CLINICAS, UNIV SAO PAULO10.6061/clinics/2013(07)19Medicine, General & Internal