Sistema FMUSP-HC: Faculdade de Medicina da Universidade de São Paulo (FMUSP) e Hospital das Clínicas da FMUSPCALANCA, NaiadeFRANCISCO, Ana Lucia NoronhaBIZINELLI, DanielaKUASNE, HellenBARROS FILHO, Mateus CamargoFLORES, Bianca Campos TroncarelliPINTO, Clovis Antonio LopesRAINHO, Claudia AparecidaSOARES, Milena Botelho PereiraMARCHI, Fabio AlbuquerqueKOWALSKI, Luiz PauloROGATTO, Silvia Regina2024-02-152024-02-152023BIOMEDICINE & PHARMACOTHERAPY, v.167, article ID 115559, 14p, 20230753-3322https://observatorio.fm.usp.br/handle/OPI/57806Oral cavity squamous cell carcinoma (OSCC) is a complex and dynamic disease characterized by clinicopathological and molecular heterogeneity. Spatial and temporal heterogeneity of cell subpopulations has been associated with cancer progression and implicated in the prognosis and therapy response. Emerging evidence indicates that aberrant epigenetic profiles in OSCC may foster an immunosuppressive tumor microenvironment by modulating the expression of immune-related long non-coding RNAs (lncRNAs). DNA methylation analysis was performed in 46 matched OSCC and normal adjacent tissue samples using a genome-wide platform (Infinium HumanMethylation450 BeadChip). Reference-based computational deconvolution (MethylCIBERSORT) was applied to infer the immune cell composition of the bulk samples. The expression levels of genes encoding immune markers and differentially methylated lncRNAs were investigated using The Cancer Genome Atlas dataset. OSCC specimens presented distinct immune cell composition, including the enrichment of monocyte lineage cells, natural killer cells, cytotoxic T-lymphocytes, regulatory T-lymphocytes, and neutrophils. In contrast, B-lymphocytes, effector T-lymphocytes, and fibroblasts were diminished in tumor samples. The hypomethylation of three immune-associated lncRNAs (MEG3, MIR155HG, and WFDC21P) at individual CpG sites was confirmed by bisulfite-pyrosequencing. Also, the upregulation of a set of immune markers (FOXP3, GZMB, IL10, IL2RA, TGFB, IFNG, TDO2, IDO1, and HIF1A) was detected. The immune cell composition, immune markers alteration, and dysregulation of immune-associated lncRNAs reinforce the impact of the immune microenvironment in OSCC. These concurrent factors contribute to tumor heterogeneity, suggesting that epiimmunotherapy could be an efficient alternative to treat OSCC.engopenAccessOral cancerTumor immune microenvironmentLncRNAsDNA methylationDeconvolutionb-cellscancerdifferentiationinducearticleCopyright ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER10.1016/j.biopha.2023.115559Medicine, Research & ExperimentalPharmacology & Pharmacy1950-6007