GIUSEPPINA MARIA PATAVINO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
5 resultados
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conferenceObject Incidence of Chagas Cardiomyopathy and Relative Diagnostic Value of Electrocardiogram (ECG) Versus Echocardiogram (ECHO) Among T. cruzi Seropositive Donors(2012) SABINO, E. C.; RIBEIRO, A.; PATAVINO, G. M.; CAPUANI, L. D.; ALMEIDA-NETO, C. de; OLIVEIRA, C. Di Lorenzo; CARRICK, D. M.; CUSTER, B.; BUSCH, M. P.; MURPHY, E. L.Background/Case Studies: Few studies have prospectively evaluated the natural history of Chagas cardiomyopathy, including disease penetrance, prognostic factors and indications for treatment. Nor has the diagnostic utility of ECHO been compared to the simpler and less costly ECG screening. Study Design/Methods: In a retrospective cohort study, T. cruzi seropositive blood donors with an index blood donation in 1996-2002 in Sao Paulo and Montes Claros, Brazil were matched to seronegative donors on index donation date, age, gender and city. 101 Chagas cardiomyopathy patients served as positive controls. In 2009-2010, all subjects underwent a health history questionnaire, medical examination, standardized ECG and ECHO. Subjects with abnormal screening EKG or ECHO were referred to a blinded panel of 3 cardiologists who adjudicated the outcome of Chagas cardiomyopathy. Diagnostic sensitivity, specifi city, positive predictive value (PPV) and negative predictive value (NPV) were calculated separately for ECHO and ECG. Results/Findings: Mean follow-up time was 10.5 years for the sero-positives and 11.1 years for the seronegatives. The sensitivity of the combined ECHO/ECG and expert panel algorithm was 98%, based on diagnoses of cardiomyopathy in 99 of 101 previously diagnosed Chagas cardiomyopathy patients, of whom 1 (1%) expert referral was triggered by ECHO alone, none by ECG alone and 98 (99%) by both ECHO and ECG. In contrast, 24 (5%) of 488 T. cruzi-seronegative control donors were falsely classifi ed as having Chagas cardiomyopathy (specifi city = 95%). Among the 499 T. cruzi seropositives, 315 (63%) were referred to the expert panel and 120 (24%) had defi nite Chagas-like cardiomyopathy, leading to an incidence difference of 1.85 per 100 person-years attributable to T. cruzi infection. The Table summarizes the sensitivity, specifi city and predictive values of ECHO and ECG screening for cardiomyopathy diagnosis among the T. cruzi seropositive individuals. Conclusion: There is a substantial (~2%) annual incidence of Chagas cardiomyopathy among initially asymptomatic T. cruzi seropositive blood donors. Among seropositives, ECG had better sensitivity and ECHO had better specifi city. Both tests had poor positive predictive value, but ECG had better negative predictive value. Future studies could use a two-step algorithm in which ECG is used to exclude those with a negative test and low likelihood of disease. ECHO would be done to refine the diagnosis only in patients with abnormal ECG fi ndings. Disclosure of Commercial Conflict of Interest M. P. Busch: NIH, Grants or Research Support; Novartis/Gen-Probe, Grants or Research Support; Terumo/Caridian, Grants or Research Support; Gen-Probe, Consulting or Board of Director Fees; Johnson & Johnson/Merck, Ortho, Consulting or Board of Director Fees; Abbott, Travel Support or Honorarium; Novartis, Travel Support or Honorarium; L. D. Capuani: Nothing to disclose; D. M. Carrick: Nothing to disclose; B. Custer: Nothing to disclose; C. de Almeida-Neto: Nothing to disclose; C. Di Lorenzo Oliveira: No Answer; E. L. Murphy: Nothing to disclose; G. M. Patavino: Nothing to disclose; A. Ribeiro: Nothing to disclose; E. C. Sabino: Nothing to disclose Disclosure of Grants Conflict of Interest M. P. Busch: Novartis, Grants or Research Support; Ortho, Grants or Research Support; Terumo/Caridian, Grants or Research Support; L. D. Capuani: Nothing to disclose; D. M. Carrick: Nothing to disclose; B. Custer: Nothing to disclose; C. de Almeida-Neto: Nothing to disclose; C. Di Lorenzo Oliveira: No Answer; E. L. Murphy: Nothing to disclose; G. M. Patavino: Nothing to disclose; A. Ribeiro: Nothing to disclose; E. C. Sabino: Nothing to disclose Expert Panel Diagnosis of Cardiomyopathy in T. cruzi seropositives ECHO YES (N = 120) NO (N = 379) Triggered by ECHO 75 77 PPV = 49% Not Triggered by ECHO 45 302 NPV = 87% Sens = 63% Spec = 80% ECG Triggered by ECG 114 151 PPV = 43% Not Triggered by ECG 6 228 NPV = 97% Sens = 95% Spec = 60%- Number of recent sexual partners among blood donors in Brazil: associations with donor demographics, donation characteristics, and infectious disease markers(2012) PATAVINO, Giuseppina Maria; ALMEIDA-NETO, Cesar de; LIU, Jing; WRIGHT, David J.; MENDRONE-JUNIOR, Alfredo; FERREIRA, Maria Ines Lopes; CARNEIRO, Anna Barbara de Freitas; CUSTER, Brian; FERREIRA, Joao Eduardo; BUSCH, Michael P.; SABINO, Ester CerdeiraBACKGROUND: Brazilian blood centers ask candidate blood donors about the number of sexual partners in the past 12 months. Candidates who report a number over the limit are deferred. We studied the implications of this practice on blood safety. STUDY DESIGN AND METHODS: We analyzed demographic characteristics, number of heterosexual partners, and disease marker rates among 689,868 donations from three Brazilian centers between July 2007 and December 2009. Donors were grouped based on maximum number of partners allowed in the past 12 months for each center. Chi-square and logistic regression analysis were conducted to examine associations between demographic characteristics, number of sex partners, and individual and overall positive markers rates for human immunodeficiency virus (HIV), human T-lymphotropic virus Types 1 and 2, hepatitis B virus, hepatitis C virus, and syphilis. RESULTS: First-time, younger, and more educated donors were associated with a higher number of recent sexual partners, as was male sex in Sao Paulo and Recife (p < 0.001). Serologic markers for HIV and syphilis and overall were associated with multiple partners in Sao Paulo and Recife (p < 0.001), but not in Belo Horizonte (p = 0.05, p = 0.94, and p = 0.75, respectively). In logistic regression analysis, number of recent sexual partners was associated with positive serologic markers (adjusted odds ratio [AOR], 1.2-1.5), especially HIV (AOR, 1.9-4.4). CONCLUSIONS: Number of recent heterosexual partners was associated with HIV positivity and overall rates of serologic markers of sexually transmitted infections. The association was not consistent across centers, making it difficult to define the best cutoff value. These findings suggest the use of recent heterosexual contacts as a potentially important deferral criterion to improve blood safety in Brazil.
- Risk factors for deferral due to low hematocrit and iron depletion among prospective blood donors in a Brazilian center(2015) DAUAR, Eloísa Tedeschi; PATAVINO, Giuseppina Maria; MENDRONE JÚNIOR, Alfredo; GUALANDRO, Sandra Fátima Menosi; SABINO, Ester Cerdeira; ALMEIDA-NETO, Cesar deOBJECTIVE: Deferral of blood donors due to low hematocrit and iron depletion is commonly reported in blood banks worldwide. This study evaluated the risk factors for low hematocrit and iron depletion among prospective blood donors in a large Brazilian blood center.METHOD: A case-control study of 400 deferred donors due to low hematocrit and 456 eligible whole blood donors was conducted between 2009 and 2011. Participants were interviewed about selected risk factors for anemia, and additional laboratory tests, including serum ferritin, were performed. Bivariate and multivariate analyses were performed to assess the association between predictors and deferral due to low hematocrit in the studied population and iron depletion in women.RESULTS: Donors taking aspirins or iron supplementation, those who reported stomachache, black tarry stools or hematochezia, and women having more than one menstrual period/month were more likely to be deferred. Risk factors for iron depletion were repeat donation and being deferred at the hematocrit screening. Smoking and lack of menstruation were protective against iron depletion.CONCLUSION: This study found some unusual risk factors related to gastrointestinal losses that were associated with deferral of donors due to low hematocrit. Knowledge of the risk factors can help blood banks design algorithms to improve donor notification and referral.
- Contribution of the Retrovirus Epidemiology Donor Study (REDS) to research on blood transfusion safety in Brazil(2014) LOUREIRO, Paula; ALMEIDA-NETO, Cesar de; PROIETTI, Anna Bárbara Carneiro; CAPUANI, Ligia; GONÇALEZ, Thelma Terezinha; OLIVEIRA, Claudia Di Lorenzo de; LEÃO, Silvana Carneiro; LOPES, Maria Inês; SAMPAIO, Divaldo; PATAVINO, Giuseppina Maria; FERREIRA, João Eduardo; BLATYTA, Paula Fraiman; LOPES, Maria Esther Duarte; MENDRONE-JUNIOR, Alfredo; SALLES, Nanci Alves; KING, Melissa; MURPHY, Edward; BUSCH, Michael; CUSTER, Brian; SABINO, Ester CerdeiraThe Retrovirus Epidemiology Donor Study (REDS) program was established in the United States in 1989 with the purpose of increasing blood transfusion safety in the context of the HIV/AIDS and human T-lymphotropic virus epidemics. REDS and its successor, REDS-II were at first conducted in the US, then expanded in 2006 to include international partnerships with Brazil and China. In 2011, a third wave of REDS renamed the Recipient Epidemiology and Donor Evaluation Study-III (REDS-III) was launched. This seven-year research program focuses on both blood banking and transfusion medicine research in the United States of America, Brazil, China, and South Africa. The main goal of the international programs is to reduce and prevent the transmission of HIV/AIDS and other known and emerging infectious agents through transfusion, and to address research questions aimed at understanding global issues related to the availability of safe blood. This article describes the contribution of REDS-II to transfusion safety in Brazil. Articles published from 2010 to 2013 are summarized, including database analyses to characterize blood donors, deferral rates, and prevalence, incidence and residual risk of the main blood-borne infections. Specific studies were developed to understand donor motivation, the impact of the deferral questions, risk factors and molecular surveillance among HIV-positive donors, and the natural history of Chagas disease. The purpose of this review is to disseminate the acquired knowledge and briefly summarize the findings of the REDS-II studies conducted in Brazil as well as to introduce the scope of the REDS-III program that is now in progress and will continue through 2018.
conferenceObject TEN-YEAR INCIDENCE OF CHAGAS CARDIOMYOPATHY AMONG ASYMPTOMATIC T. CRUZI SEROPOSITIVE, FORMER BLOOD DONORS(2012) MURPHY, E. L.; SABINO, E.; RIBEIRO, A.; SALEMI, V; ANTUNES, A.; MENEZES, M.; IANNI, B.; NASTARI, L.; FERNANDEZ, F.; PATAVINO, G.; SACHDEV, V; CAPUANI, L.; ALMEIDA NETO, C. De; OLIVEIRA, C. Di Lorenzo; CARRICK, D.; WRIGHT, D.; CUSTER, B.; BUSCH, M. P.; MURPHY, E. L.