DANIEL REIS WAISBERG

(Fonte: Lattes)
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Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado, Hospital das Clínicas, Faculdade de Medicina

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  • conferenceObject
    Innovative technique avoiding bleeding after reperfusion in living donor liver transplatation using a modified right lobe graft
    (2021) PINHEIRO, R. S.; ANDRAUS, W.; NACIF, L. S.; MARTINO, R. B.; DUCATTI, L.; ARANTES, R. M.; WAISBERG, D. R.; FORTUNATO, A.; ROCHA-SANTOS, V.; D'ALBUQUERQUE, L. C.
  • conferenceObject
    Translational Research for Pelvic Floor Transplantation
    (2017) GALVAO, Flavio; WAISBERG, Daniel; SEID, Victor; CRUZ, Ruy; TRALDI, Maria; ARAUJO, Bruno; PANTANALI, Carlos; ANDRAUS, Wellington; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz
  • article 11 Citação(ões) na Scopus
    Glycomics expression analysis of sulfated glycosaminoglycans of human colorectal cancer tissues and non-neoplastic mucosa by electrospray ionization mass spectrometry
    (2015) MAROLLA, Ana Paula Cleto; WAISBERG, Jaques; SABA, Gabriela Tognini; WAISBERG, Daniel Reis; MARGEOTTO, Fernando Beani; PINHAL, Maria Aparecida da Silva
    ABSTRACT Objective To determine the presence of glycosaminoglycans in the extracellular matrix of connective tissue from neoplastic and non-neoplastic colorectal tissues, since it has a central role in tumor development and progression. Methods Tissue samples from neoplastic and non-neoplastic colorectal tissues were obtained from 64 operated patients who had colorectal carcinoma with no distant metastases. Expressions of heparan sulphate, chondroitin sulphate, dermatan sulphate and their fragments were analyzed by electrospray ionization mass spectrometry, with the technique for extraction and quantification of glycosaminoglycans after proteolysis and electrophoresis. The statistical analysis included mean, standard deviation, and Student’st test. Results The glycosaminoglycans extracted from colorectal tissue showed three electrophoretic bands in agarose gel. Electrospray ionization mass spectrometry showed characteristic disaccharide fragments from glycosaminoglycans, indicating their structural characterization in the tissues analyzed. Some peaks in the electrospray ionization mass spectrometry were not characterized as fragments of sugars, indicating the presence of fragments of the protein structure of proteoglycans generated during the glycosaminoglycan purification. The average amount of chondroitin and dermatan increased in the neoplastic tissue compared to normal tissue (p=0.01). On the other hand, the average amount of heparan decreased in the neoplastic tissue compared to normal tissue (p= 0.03). Conclusion The method allowed the determination of the glycosaminoglycans structural profile in colorectal tissue from neoplastic and non-neoplastic colorectal tissue. Neoplastic tissues showed greater amounts of chondroitin sulphate and dermatan sulphate compared to non-neoplastic tissues, while heparan sulphate was decreased in neoplastic tissues.
  • conferenceObject
    Hyperacute Rejection in Multivisceral Xenotransplantation
    (2012) GALVAO, F.; SOLER, W.; POMPEU, E.; COSTA, A.; WAISBERG, D.; CAPELLOZI, V.; TEODORO, W.; VELOSA, A.; CHAIB, E.; D'ALBUQUERQUE, L.
  • article 0 Citação(ões) na Scopus
    Innovative Technique Avoiding Bleeding After Reperfusion in Living Donor Liver Transplant Using a Modified Right Lobe Graft
    (2022) PINHEIRO, Rafael S.; ANDRAUS, Wellington; FORTUNATO, Allana C.; FERNANDES, Michel Ribeiro; NACIF, Lucas Souto; MARTINO, Rodrigo Bronze De; DUCATTI, Liliana; WAISBERG, Daniel Reis; ARANTES, Rubens Macedo; ROCHA-SANTOS, Vinicius; GALVAO, Flavio Henrique Ferreira; CARNEIRO-D'ALBUQUERQUE, Luiz Augusto
    Background. Living donor liver transplant (LDLT) is a valuable therapeutic option for over-coming the deceased donor shortage. Modified right lobe graft (MRLG) keeps the middle hepatic vein (MHV) trunk with the remnant liver to improve donor safety. Hemostasis in the MHV tributary reconstruction can be tricky; surgical stitches and energy coagulation are ineffective. Fibrin glues are excellent vascular sealants but are poor in maintaining hemostasis in an active hemorrhage or preventing resection surface-related complications after liver resection. We propose applying fibrin sealant during back table graft preparation to seal the hepatic edge and MHV reconstruction to avoid bleeding after graft revascularization. Methods. Our retrospective cohort study included all adult patients undergoing LDLT between August 2017 and December 2021. During the back table procedure, we performed the reconstruction of the inferior right hepatic vein and/or MHV tributaries from segment 5 (V5) and segment 8 (V8) using a vein harvested from a nonrelated deceased donor. Before initiating the hepatic graft implantation, we applied fibrin sealant in the resected parenchyma, especially in the V5 and V8 anastomosis, to seal the hepatic edge and hepatic vein reconstruction. Results. No bleeding was identified in the hepatic edge, and blood product transfusion was unnecessary for any recipients after reperfusion. Conclusion. In LDLT using MRLG with MHV reconstruction, the fibrin sealant, when applied on the raw hepatic surface, and vascular reconstruction during back table graft preparation avoided bleeding after graft revascularization.
  • article 4 Citação(ões) na Scopus
    Novel Technique in a Sheep Model of Uterine Transplantation
    (2020) ARANTES, Rubens Macedo; NACIF, Lucas Souto; PINHEIRO, Rafael Soares; ROCHA-SANTOS, Vinicius; MARTINO, Rodrigo Bronze de; WAISBERG, Daniel Reis; PANTANALI, Carlos Andres Rodriguez; FORTUNATO, Allana; LIMA, Marisa Rafaela; DUCATTI, Liliana; HADDAD, Luciana Bertocco de Paiva; EJZENBERG, Dani; GALVAO, Flavio Henrique; ANDRAUS, Wellington; CARNEIRO-D'ALBUQUERQUE, Luiz
  • conferenceObject
    Pelvic Floor Transplantation in The Reat.
    (2014) GALVAO, F.; BAPTISTA, R.; SEID, V.; WAISBERG, D.; RODRIGUES, P.; CHAIB, E.; D'ALBUQUERQUE, L.
  • conferenceObject
    The Role of the Social Worker Concerning Intestinal Rehabilitation and Transplantation in a Latin American Center
    (2021) GALVAO, F.; LEE, A.; TANJI, J.; RANA, T.; DUARTE, M.; SCABIA, T.; WAISBERG, D.; ROCHA, M.; PINHEIRO, R.; CARNEIRO-D'ALBUQUERQUE, L.
  • conferenceObject
    Adequate Exposure to Tacrolimus with Sublingual Administration
    (2021) MACIEL, A.; FORTUNATO, A.; WAISBERG, D.; GALVAO, F.; LEE, A.; ARANTES, Jr R.; ROCHA, M.; TANIGAWA, R.; DUCATTI, L.; MARTINO, R.; NACIF, L.; ROCHA-SANTOS, V; ANDRAUS, W.; D'ALBUQUERQUE, L.
  • article 8 Citação(ões) na Scopus
    Desmoid tumors of the chest wall: surgical challenges and possible risk factors
    (2011) ABRAO, Fernando Conrado; WAISBERG, Daniel Reis; FERNANDEZ, Angelo; BERNARDO, Wanderley Marques; PEGO-FERNANDES, Paulo Manuel; JATENE, Fabio Biscegli