CAMILA GALVAO LOPES
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- Intestinal Translocation of Clinical Isolates of Vancomycin-Resistant Enterococcus faecalis and ESBL-Producing Escherichia coli in a Rat Model of Bacterial Colonization and Liver Ischemia/Reperfusion Injury(2014) HEIJDEN, Karin M. van der; HEIJDEN, Inneke M. van der; GALVAO, Flavio H.; LOPES, Camila G.; COSTA, Silvia F.; ABDALA, Edson; D'ALBUQUERQUE, Luiz A.; LEVIN, Anna S.The objectives of this study were to develop a rat model of gastrointestinal colonization with vancomycin-resistant Enterococcus faecalis (VRE) and extended-spectrum beta-lactamase (ESBL)-producing E. coli and to evaluate intestinal translocation to blood and tissues after total and partial hepatic ischemia. Methods - We developed a model of rat colonization with VRE and ESBL-E coli. Then we studied four groups of colonized rats: Group I (with hepatic pedicle occlusion causing complete liver ischemia and intestinal stasis); Group II (with partial liver ischemia without intestinal stasis); Group III (surgical manipulation without hepatic ischemia or intestinal stasis); Group IV (anesthetized without surgical manipulation). After sacrifice, portal and systemic blood, large intestine, small intestine, spleen, liver, lungs, and cervical and mesenteric lymph nodes were cultured. Endotoxin concentrations in portal and systemic blood were determined. Results - The best inocula were: VRE: 2.4x10(10) cfu and ESBL-E. coli: 1.12x10(10) cfu. The best results occurred 24 hours after inoculation and antibiotic doses of 750 mu mg/mL of water for vancomycin and 2.1 mg/mL for ceftriaxone. There was a significantly higher proportion of positive cultures for ESBL-E. coli in the lungs in Groups I, II and III when compared with Group IV (67%; 60%; 75% and 13%, respectively; p: 0.04). VRE growth was more frequent in mesenteric lymph nodes for Groups I (67%) and III (38%) than for Groups II (13%) and IV (none) (p:0.002). LPS was significantly higher in systemic blood of Group I (9.761x13.804 EU/mL-p:0.01). No differences for endotoxin occurred in portal blood. Conclusion - e developed a model of rats colonized with resistant bacteria useful to study intestinal translocation. Translocation occurred in surgical procedures with and without hepatic ischemia-reperfusion and probably occurred via the bloodstream. Translocation was probably lymphatic in the ischemia-reperfusion groups. Systemic blood endotoxin levels were higher in the group with complete hepatic ischemia.
- Unilateral non traumatic vertebral artery dissection with cervical spinal cord infarction(2012) TAKAHASHI, Patricia Gushiken; CURY, Rubens Gisbert; LOPES, Camila Galvao; SIMABUKURO, Mateus Mistieri; MARCHIORI, Paulo Euripedes
- Conceptual and Operational Definitions of the Defining Characteristics and Related Factors of the Diagnosis Ineffective Health Management in People With Heart Failure(2017) CARNEIRO, Camila de Souza; LOPES, Camila Takao; LOPES, Juliana de Lima; SANTOS, Vinicius Batista; BACHION, Maria Marcia; BARROS, Alba Lucia Bottura Leite deObjectiveTo construct conceptual and operational definitions for the defining characteristics (DCs) and related factors (RFs) of the nursing diagnosis (ND) ineffective health management for people with chronic heart failure. MethodConceptual and operational definitions for the DC and RF were based on studies found in an integrative literature review in the databases Latin American Literature in Health Sciences, Cumulative Index to Nursing and Allied Health Literature, and MEDLINE by using the key words Nursing diagnosis, Heart Failure, and Patient Cooperation in different combinations. ResultsConceptual and operational definitions for all the DCs and RFs were constructed. Conclusions and Implications for PracticeThe definitions will facilitate in the identification of ND in clinical practice in patients with heart failure, future research on ND validation, and education in undergraduate courses. ObjetivoConstruir definicoes conceituais e operacionais para as caracteristicas definidoras (CD) e fatores relacionados (FR) do diagnostico de enfermagem (DE) Controle Ineficaz da Saude para pessoas com insuficiencia cardiaca. MetodoDefinicoes conceituais e operacionais das CD e FR basearam-se em estudos encontrados em uma revisAo integrativa de literatura nas bases de dados Lilacs, CINAHL e Medline utilizando as palavras-chave Nursing diagnosis, Heart Failure, e Patient Cooperation em diferentes combinacoes. ResultadosForam construidas definicoes conceituais e operacionais para todas as DC e FR. Conclusoes e Implicacoes para a praticaAs definicoes facilitarAo a identificacAo do DE na pratica clinica em pacientes com insuficiencia cardiaca, futuras pesquisas de validacAo do DE e ensino nos cursos de graduacAo.
- Genetic analysis of patients with familial and sporadic amyotrophic lateral sclerosis in a Brazilian Research Center(2017) CHADI, Gerson; MAXIMINO, Jessica Ruivo; JORGE, Frederico Mennucci De Haidar; BORBA, Fabricio Castro De; GILIO, Joyce Meire; CALLEGARO, Dagoberto; LOPES, Camila Galvao; SANTOS, Samantha Nakamura Dos; REBELO, Gabriela Natania SalesObjective: To investigate gene mutations in familial form (FALS) and sporadic form (SALS) of amyotrophic lateral sclerosis (ALS) in a highly miscegenated population. Methods: Frequencies of mutations in the C9orfF72, TARDBP, SOD1, FUS and VAPB genes were investigated in a cohort of FALS (n=39) and SALS (n=189) subjects from the Research Centre of the University of SAo Paulo School of Medicine. All patients were subjected to C9orf72 and TARDBP analyses. SOD1, FUS and VAPB were also evaluated in FALS subjects. Results: Mutations were identified in FALS (61.3%) and SALS (5.3%) patients. Mutations in C9orf72 (12.8%,>45 GGGGCC hexanucleotide repeats), VAPB (43.6%, P56S) and SOD1 (7.7%, L145S) were identified in FALS subjects. Pathogenic C9orf72 expansions (2.64%) were identified in some SALS patients. Similar changes of TARDBP were found in SALS (2.64%) but not in FALS subjects. No FUS mutations were seen in any FALS subjects. Conclusions: TARDBP and C9orf72 mutations in this cohort were similar to those found in other centres worldwide. VAPB mutation (P56S) was highly prevalent in Brazilian FALS patients.