RICARDO JOSE RODRIGUEZ FERREIRA
Índice h a partir de 2011
5
Projetos de Pesquisa
Unidades Organizacionais
Instituto de Ortopedia e Traumatologia, Hospital das Clínicas, Faculdade de Medicina - Médico
16 resultados
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Agora exibindo 1 - 10 de 16
- Effects of estrogen on functional and neurological recovery after spinal cord injury: An experimental study with rats(2015) LETAIF, Olavo Biraghi; CRISTANTE, Alexandre Fogaca; BARROS FILHO, Tarcisio Eloy Pessoa de; FERREIRA, Ricardo; SANTOS, Gustavo Bispo dos; ROCHA, Ivan Dias da; MARCON, Raphael MartusOBJECTIVES: To evaluate the functional and histological effects of estrogen as a neuroprotective agent after a standard experimentally induced spinal cord lesion. METHODS: In this experimental study, 20 male Wistar rats were divided into two groups: one group with rats undergoing spinal cord injury (SCI) at T10 and receiving estrogen therapy with 17-beta estradiol (4mg/kg) immediately following the injury and after the placement of skin sutures and a control group with rats only subjected to SCI. A moderate standard experimentally induced SCI was produced using a computerized device that dropped a weight on the rat's spine from a height of 12.5 mm. Functional recovery was verified with the Basso, Beattie and Bresnahan scale on the 2nd, 7th, 14th, 21st, 28th, 35th and 42nd days after injury and by quantifying the motor-evoked potential on the 42nd day after injury. Histopathological evaluation of the SCI area was performed after euthanasia on the 42nd day. RESULTS: The experimental group showed a significantly greater functional improvement from the 28th to the 42nd day of observation compared to the control group. The experimental group showed statistically significant improvements in the motor-evoked potential compared with the control group. The results of pathological histomorphometry evaluations showed a better neurological recovery in the experimental group, with respect to the proportion and diameter of the quantified nerve fibers. CONCLUSIONS: Estrogen administration provided benefits in neurological and functional motor recovery in rats with SCI beginning at the 28th day after injury.
- Tethered Cord Syndrome(2022) AMARAL, V. C. G.; CAVALHEIRO, S.; FERREIRA, R. J. R.; MENDONçA, M. L. F. deTethered cord syndrome is characterized by the inability to move the spinal cord during the different stages of life. Clinically, the syndrome is associated with a progressive neurological, orthopedic, and urological deterioration and misplacement of the conus medullaris. Open and closed spinal dysraphisms have a high incidence of tethered spinal cord. Surgical treatment as well as a multimodal intraoperative neurophysiological monitoring protocol, for open and closed spinal dysraphisms, during fetal and postnatal stages is described in detail in this chapter. A mutual understanding between the neurosurgeon, the anesthesiologist, and the neurophysiologist is necessary for minimizing deficits postoperative. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.
- Neuroprotective effect of epidural hypothermia after spinal cord lesion in rats(2014) BARBOSA, Marcello Oliveira; CRISTANTE, Alexandre Fogaca; SANTOS, Gustavo Bispo dos; FERREIRA, Ricardo; MARCON, Raphael Martus; BARROS FILHO, Tarcisio Eloy Pessoa deOBJECTIVES : To evaluate the neuroprotective effect of epidural hypothermia in rats subjected to experimental spinal cord lesion. METHODS: Wistar rats (n = 30) weighing 320-360 g were randomized to two groups (hypothermia and control) of 15 rats per group. A spinal cord lesion was induced by the standardized drop of a 10-g weight from a height of 2.5 cm, using the New York University Impactor, after laminectomy at the T9-10 level. Rats in the hypothermia group underwent epidural hypothermia for 20 minutes immediately after spinal cord injury. Motor function was assessed for six weeks using the Basso, Beattie and Bresnahan motor scores and the inclined plane test. At the end of the final week, the rats' neurological status was monitored by the motor evoked potential test and the results for the two groups were compared. RESULTS: Analysis of the Basso, Beattie and Bresnahan scores obtained during the six-week period indicated that there were no significant differences between the two groups. There was no significant difference between the groups in the inclined plane test scores during the six-week period. Furthermore, at the end of the study, the latency and amplitude values of the motor evoked potential test were not significantly different between the two groups. CONCLUSION: Hypothermia did not produce a neuroprotective effect when applied at the injury level and in the epidural space immediately after induction of a spinal cord contusion in Wistar rats.
- Evaluation of the effects of erythropoietin and interleukin-6 in rats submitted to acute spinal cord injury(2019) BARROS, Alderico Girao Campos de; CRISTANTE, Alexandre Fogaca; SANTOS, Gustavo Bispo dos; NATALINO, Renato Jose Mendonca; FERREIRA, Ricardo Jose Rodriguez; BARROS-FILHO, Tarcisio Eloy Pessoa DeOBJECTIVE: To evaluate the effects of interleukin-6 (IL-6) and erythropoietin (EPO) in experimental acute spinal cord injury (SCI) in rats. METHODS: Using standardized equipment, namely, a New York University (NYU) Impactor, a SCI was produced in 50 Wistar rats using a 10-g weight drop from a 12.5-mm height. The rats were divided into the following 5 groups of 10 animals each: ""Group EPO"", treated with erythropoietin only; ""Group EPO + IL-6"", treated with both substances; ""Group IL-6"", receiving IL-6 administration only; ""Group Placebo"", receiving a placebo solution; and ""Group Sham"", submitted to an incomplete procedure (only laminectomy, without SCI). All drugs and the placebo solution were administered intraperitoneally for three weeks. The animals were followed up for 42 days. Functional motor recovery was monitored by the Basso, Beattie, and Bresnahan (BBB) scale on days 2, 7, 14, 21, 28, 35 and 42. Motor-evoked potential tests were performed on the 42nd day. Histological analysis was performed after euthanasia. RESULTS: The group receiving EPO exhibited superior functional motor results on the BBB scale. IL-6 administration alone was not superior to the placebo treatment, and the IL-6 combination with EPO yielded worse results than did EPO alone. CONCLUSIONS: Using EPO after acute SCI in rats yielded benefits in functional recovery. The combination of EPO and IL-6 showed benefits, but with inferior results compared to those of isolated EPO; moreover, isolated use of IL-6 resulted in no benefit.
- Neurophysiological assessment of spinal cord injuries in dogs using somatosensory and motor evoked potentials(2017) SOUZA, Maria Claudia Campos Mello Inglez de; FERREIRA, Ricardo Jose Rodriguez; PATRICIO, Geni Cristina Fonseca; MATERA, Julia MariaSomatosensory evoked potentials (SSEPs) and motor evoked potentials (MEPs) are non-invasive neurophysiological tests that reflect the functional integrity of sensory and motor pathways. Despite their extensive use and description in human medicine, reports in veterinary medicine are scarce. SSEPs are obtained via peripheral stimulation of sensory or mixed nerves; stimulation induces spinal and cortical responses, which are recorded when sensory pathways integrity is preserved. MEPs can be obtained via transcranial electrical or magnetic stimulation; in this case, thoracic and pelvic limb muscle responses are captured if motor pathways are preserved. This review describes principles, methodology and clinical applicability of SSEPs and MEPs in companion animal medicine. Potential interferences of anesthesia with SSEP and MEP recording are also discussed.
- Standardization of an experimental model of intradural injection after spinal cord injury in rats(2021) LETAIF, Olavo B.; TAVARES-JUNIOR, Mauro C. M.; SANTOS, Gustavo B. dos; FERREIRA, Ricardo J. R.; MARCON, Raphael M.; CRISTANTE, Alexandre F.; BARROS-FILHO, Tarcisio E. P. deOBJECTIVES: The intrathecal route has not yet been thoroughly standardized and evaluated in an experimental model of spinal cord injury (SCI) in Wistar rats. The objective of this study was to standardize and evaluate the effect of intradural injection in this animal model. METHOD: The animals were divided into 6 groups: 1) laminectomy and intradural catheter; 2) laminectomy, intradural catheter and infusion; 3) only SCI; 4) SCI and intradural catheter; 5) SCI, intradural catheter and infusion; and 6) control (laminectomy only). Motor evaluations were performed using the Basso, Beattie and Bresnahan (BBB) scale and the horizontal ladder test; motor evoked potentials were measured for functional evaluation, and histological evaluation was performed as well. All experimental data underwent statistical analysis. RESULTS: Regarding motor evoked potentials, the groups with experimental SCI had worse results than those without, but neither dural puncture nor the injection of intrathecal solution aggravated the effects of isolated SCI. Regarding histology, adverse tissue effects were observed in animals with SCI. On average, the BBB scores had the same statistical behaviour as the horizontal ladder results, and at every evaluated timepoint, the groups without SCI presented scored significantly better than those with SCI (p<0.05). The difference in performance on motor tests between rats with and without experimental SCI persisted from the first to the last test. CONCLUSIONS: The present work standardizes the model of intradural injection in experimental SCI in rats. Intrathecal puncture and injection did not independently cause significant functional or histological changes.
- Effects of tacrolimus and erythropoietin in experimental spinal cord lesion in rats: functional and histological evaluation(2016) COUTINHO, P. R. de Mesquita; CRISTANTE, A. F.; BARROS FILHO, T. E. P. de; FERREIRA, R.; SANTOS, G. B. dosStudy design: Experimental study with rats. Objective: To evaluate functional and histological effects of tacrolimus (FK 506) and erythropoietin (EPO) after experimental spinal cord contusion injury (SCI). Setting: Brazil. Methods: Wistar rats (n=60) were submitted to SCI with the NYU Impactor system. The control group received saline; the EPO group received EPO; the group EPO+FK 506 received EPO associated with tacrolimus and the group FK 506 received tacrolimus only. The Sham group underwent SCI, but did not receive any drug. Locomotor function was evaluated after SCI by BBB (Basso, Beattie and Bresnahan) weekly and by the motor-evoked potential test in 42 days. The spinal cord was histologically evaluated. Results: There was a significant difference between treated and the control groups from the seventh day on for BBB scores, with no difference between the groups EPO and EPO+FK 506 by the end of the study. There were significant differences between groups for necrosis and bleeding, but not for hiperemia, degeneration and cellular infiltrate. Axon neuron count was different between all groups (P=0.001), between EPO+FK 506 and FK 506 (P=0.011) and between EPO+FK 506 and Sham (P=0.002). Amplitude was significantly different between all groups except between control and sham. For latency, there was no difference. Conclusions: This study did not reveal significant differences in the recovery of locomotor function, or in the histological and electrophysiological analysis in animals treated with EPO and tacrolimus after thoracic SCI.
- Cervical Spinal Surgery(2022) FERREIRA, R. J. R.; TOSCANO, P.; BORDINI, E. C.; POETSCHER, A. W.One of the main applications of multimodal IONM is cervical spine surgery. Its importance begins in the preoperative period, with the evaluation of the correct positioning of the patient. IONM diagnoses pre-existing neurological deficits and helps to prevent central and peripheral nervous system injuries. In this chapter, IONM in decompressive cervical spine surgery is initially addressed from the surgeon’s perspective, with the relation of the main surgical times. We describe the approach routes in cervical decompression surgeries, their main risks from a neurological point of view, and the tests that should be used in IONM, in a suggested “step by step” protocol. The evidence for the use of IONM, as well as its potential benefits, during cervical spine surgery is discussed. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.
- Mandibular Branch of the Facial Nerve in Wistar Rats: New Experimental Model to Assess Facial Nerve Regeneration(2014) BENTO, Ricardo Ferreira; SALOMONE, Raquel; NASCIMENTO, Silvia Bona do; FERREIRA, Ricardo Jose Rodriguez; SILVA, Ciro Ferreira da; COSTA, Heloisa Juliana Zabeu RossiIntroduction The ideal animal model for nerve regeneration studies is the object of controversy, because all models described by the literature have advantages and disadvantages. Objective To describe the histologic and functional patterns of the mandibular branch of the facial nerve of Wistar rats to create a new experimental model of facial nerve regeneration. Methods Forty-two male rats were submitted to a nerve conduction test of the mandibular branch to obtain the compound muscle action potential. Twelve of these rats had the mandibular branch surgically removed and submitted to histologic analysis (number, partial density, and axonal diameter) of the proximal and distal segments. Results There was no statistically significant difference in the functional and histologic variables studied. Conclusion These new histologic and functional standards of the mandibular branch of the facial nerve of rats establish an objective, easy, and greatly reproducible model for future facial nerve regeneration studies.
- IOM in Pelvic Floor: Gynecological and Urological Surgeries(2022) FERREIRA, R. J. R.; BORDINI, E. C.; MARTíN-PALOMEQUE, G.; CABAñES-MARTINEZ, L.; LEMOS, N. L. De Barros MoreiraPelvic floor surgeries such as gynecological and urological approaches pose risks of neural injury to structures of the somatic and autonomic nervous systems. Usually, they are radical approaches aiming to cure the patient. Neurological deficits cause an impact on patients’ quality of life due to sexual dysfunction, and urinary and fecal incontinence. Currently, there is a greater concern with their morbidity and attempts to avoid these sequelae. Intraoperative monitoring (IOM) emerges in this scenario to promote neural protection of the somatic and autonomic nervous systems. This chapter discusses the main peculiarities of IOM tests for the somatic and autonomic nervous systems in pelvic floor surgeries (gynecological and urological), considering their performance from a practical point of view. The main limitations and difficulties of IOM of the autonomic nervous system are also addressed. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022.