DANIELLE CRISTINA FONSECA CANDIAN
Projetos de Pesquisa
Unidades Organizacionais
LIM/35 - Laboratório de Nutrição e Cirurgia Metabólica do Aparelho Digestivo, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- Type 2 Diabetes Remission After Roux-en-Y Gastric Bypass: Evidence for Increased Expression of Jejunal Genes Encoding Regenerating Pancreatic Islet-Derived Proteins as a Potential Mechanism(2017) SALA, Priscila; TORRINHAS, Raquel Susana; FONSECA, Danielle Cristina; HEYMSFIELD, Steven; GIANNELLA-NETO, Daniel; WAITZBERG, Dan LinetzkyBackground Intestinal expression of regenerating pancreatic islet-derived protein-encoding genes (REG) would be enhanced after Roux-en-Y gastric bypass (RYGB) and would affect postoperative type 2 diabetes remission (T2Dr). Methods Intestinal biopsy samples were collected from 20 adult obese women with T2D before and 3 months after RYGB. Levels of REG expression and the gene encoding its putative receptor (EXTL3) were assessed by microarray and validated by quantitative RT-PCR. T2Dr was assessed according to ADA criteria 1 year after RYGB. Results After RYGB, only patients with T2Dr had significantly increased REG1 alpha and REG3 gamma expression in the jejunum, as validated by quantitative RT-PCR. Conclusions Our data provide support for the hypothesis that increased jejunal expression of REG genes after RYGB affects T2Dr, possibly by playing an endocrine function.
conferenceObject CHANGES IN INTESTINAL GENE EXPRESSION AFTER ROUX-EN-Y GASTRIC BYPASS COULD CONTRIBUTE TO ALZHEIMER DISEASE (AD) RISK REDUCTION.(2016) SALA, P.; BELARMINO, G.; MACHADO, N. M.; FONSECA, D. C.; ISHIDA, R. K.; TORRINHAS, R. S. M. M.; GIANNELLA-NETO, D.; WAITZBERG, D. L.conferenceObject INCREASED GHRELIN LEVELS AFTER ROUX-EN-Y GASTRIC BYPASS (RYGB) IS ASSOCIATED WITH AN UPREGULATION OF GHRELIN (GHRL) GENE EXPRESSION IN EXCLUDED STOMACH IN HUMANS(2016) FONSECA, D. C.; SALA, P.; BELARMINO, G.; MACHADO, N. M.; ISHIDA, R. K.; TORRINHAS, R. S. M. M.; GIANNELLA-NETO, D.; WAITZBERG, D. L.- Upregulation of Ghrelin Gene Expression in the Excluded Stomach of Obese Women with Type 2 Diabetes After Roux-en-Y Gastric Bypass in the SURMetaGIT Study(2018) FONSECA, Danielle Cristina; SALA, Priscila; SINGER, Joelle; SINGER, Pierre; TORRINHAS, Raquel Susana; WAITZBERG, Dan LinetzkyMechanisms of type 2 diabetes remission (T2Dr) after Roux-en-Y gastric bypass (RYGB) in obese patients appear to involve gastrointestinal hormones. The objective of this study is to explore changes in ghrelin plasma levels and ghrelin gastrointestinal gene expression (GHRL) after RYGB, and their relationships to T2Dr. In 20 obese women with T2D, before and 3 months after RYGB, we assessed GHRL expression by microarray and quantitative RT-PCR in gastrointestinal biopsy samples and plasma levels of ghrelin. After RYGB, GHRL expression increased in the excluded stomach (p < 0.05) with no change in other gastrointestinal sites. There were no significant changes in ghrelin plasma levels and no correlations with T2Dr. After RYGB, over-expression of GHRL gene occurs only in the excluded stomach with no correlation to T2Dr.
conferenceObject COMPARATIVE PERFORMANCE OF TWO METHODS FOR THE DIAGNOSIS OF METABOLIC SYNDROME (METS) IN OBESE BRAZILIAN AND CORRELATION WITH EDMONTON OBESITY STAGING SYSTEM (EOSS)(2016) SALA, P.; BELARMINO, G.; MACHADO, N. M.; TESSER, A.; FONSECA, D. C.; TORRINHAS, R. S. M. M.; GIANNELLA-NETO, D.; WAITZBERG, D. L.