BEATRIZ MANGUEIRA SARAIVA RAMANHOLO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
11 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 11
- Analysis of the inflammatory profile of children with controlled and uncontrolled severe asthma(2016) ELLER, Miriam; VERGANI, Karina; SARAIVA-ROMANHOLO, Beatriz; ANTONANGELO, Leila; MAUAD, Thais; RODRIGUES, Joaquim
- Effects of environmental exposure to iron powder in an elastase mice model(2022) GALLI, T. Tafarel; CAMPOS, E. C.; SANTOS, T. M.; FUKUZAKI, S.; CAMARGO, L. N.; BEZERRA, S. K. M.; HAMAGUCHI, S. S. S.; SILVA, F. J. A. Da; SARAIVA-ROMANHOLO, B. M.; OLIVO, C. R.; PRADO, C. M.; LOPES, F. Degobbi Tenorio Quirino Dos Santos; LEICK, E. A.; BOUROTTE, C. L. M.; BENSENOR, I. J. M.; LOTUFO, P. A.; RIGHETTI, R. F.; TIBERIO, I. F. L. C.
conferenceObject Lung inflammation was attenuated by sakuranetin treatment in a model of acute lung injury(2014) MERNAK, Marcia; SANTANA, Fernanda; PINHEIRO, Nathalia; SARAIVA-RAMANHOLO, Beatriz; GRECCO, Simone; TIBERIO, Iolanda; MARTINS, Milton; LAGO, Joao; PRADO, Carla- Occupational asthma and allergic asthma: differences in the physical activity level, clinical control, and airway inflammation(2023) SILVA, Ronaldo Aparecido Da; FREITAS, Lucas Rodrigues Silva; ALMEIDA, Francine Maria De; GALVAO, Clovis Eduardo Santos; NOGUEIRA, Soraia Felix; BEZERRA, Suellen Karoline Moreira; CARVALHO, Celso Ricardo Fernades De; ARAUJO, Kaique Alves De; CRUZ, Fabiola Matos Da; AGONDI, Rosana Camara; MARTINS, Milton Arruda; TIBERIO, Iolanda De Fatima Lopes Calvo; SARAIVA-ROMANHOLO, Beatriz Mangueira
- Investigating the Effects of a New Peptide, Derived from the Enterolobium contortisiliquum Proteinase Inhibitor (EcTI), on Inflammation, Remodeling, and Oxidative Stress in an Experimental Mouse Model of Asthma-Chronic Obstructive Pulmonary Disease Overlap (ACO)(2023) BARBOSA, Jessica Anastacia Silva; SILVA, Luana Laura Sales da; JOAO, Juliana Morelli Lopes Goncalves; CAMPOS, Elaine Cristina de; FUKUZAKI, Silvia; CAMARGO, Leandro do Nascimento; SANTOS, Tabata Maruyama dos; SANTOS, Henrique Tibucheski dos; BEZERRA, Suellen Karoline Moreira; SARAIVA-ROMANHOLO, Beatriz Mangueira; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; BONTURI, Camila Ramalho; OLIVA, Maria Luiza Vilela; LEICK, Edna Aparecida; RIGHETTI, Renato Fraga; TIBERIO, Iolanda de Fatima Lopes CalvoThe synthesized peptide derived from Enterolobium contortisiliquum (pep3-EcTI) has been associated with potent anti-inflammatory and antioxidant effects, and it may be a potential new treatment for asthma-COPD overlap-ACO). Purpose: To investigate the primary sequence effects of pep3-EcTI in an experimental ACO. BALB/c mice were divided into eight groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep3-EcTI (treated with inhibitor), ACO-DX (treated with dexamethasone), ACO-DX-pep3-EcTI (treated with dexamethasone and inhibitor), and SAL-pep3-EcTI (saline group treated with inhibitor). We evaluated the hyperresponsiveness to methacholine, exhaled nitric oxide, bronchoalveolar lavage fluid (BALF), mean linear intercept (Lm), inflammatory markers, tumor necrosis factor (TNF-alpha), interferon (IFN)), matrix metalloproteinases (MMPs), growth factor (TGF-beta), collagen fibers, the oxidative stress marker inducible nitric oxide synthase (iNOS), transcription factors, and the signaling pathway NF-kappa B in the airways (AW) and alveolar septa (AS). Statistical analysis was conducted using one-way ANOVA and t-tests, significant when p < 0.05. ACO caused alterations in the airways and alveolar septa. Compared with SAL, ACO-pep3-EcTI reversed the changes in the percentage of resistance of the respiratory system (%Rrs), the elastance of the respiratory system (%Ers), tissue resistance (%Gtis), tissue elastance (%Htis), airway resistance (%Raw), Lm, exhaled nitric oxide (ENO), lymphocytes, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, TNF-alpha, INF-gamma, MMP-12, transforming growth factor (TGF)-beta, collagen fibers, and iNOS. ACO-DX reversed the changes in %Rrs, %Ers, %Gtis, %Htis, %Raw, total cells, eosinophils, neutrophils, lymphocytes, macrophages, IL-1 beta, IL-6, IL-10, IL-13, IL-17, TNF-alpha, INF-gamma, MMP-12, TGF-beta, collagen fibers, and iNOS. ACO-DX-pep3-EcTI reversed the changes, as was also observed for the pep3-EcTI and the ACO-DX-pep3-EcTI. Significance: The pep3-EcTI was revealed to be a promising strategy for the treatment of ACO, asthma, and COPD.
- Effects of a Peptide Derived from the Primary Sequence of a Kallikrein Inhibitor Isolated from Bauhinia bauhinioides (pep-BbKI) in an Asthma-COPD Overlap (ACO) Model(2023) SILVA, Luana Laura Sales da; BARBOSA, Jessica Anastacia Silva; JOAO, Juliana Morelli Lopes Goncalves; FUKUZAKI, Silvia; CAMARGO, Leandro do Nascimento; SANTOS, Tabata Maruyama dos; CAMPOS, Elaine Cristina de; COSTA, Arthur Silva; SARAIVA-ROMANHOLO, Beatriz Mangueira; BEZERRA, Suellen Karoline Moreira; LOPES, Fernanda Tenorio Quirino dos Santos; BONTURI, Camila Ramalho; OLIVA, Maria Luiza Vilela; LEICK, Edna Aparecida; RIGHETTI, Renato Fraga; TIBERIO, Iolanda de Fatima Lopes Calvo(1) There are several patients with asthma-COPD overlap (ACO). A peptide derived from the primary sequence of a kallikrein inhibitor isolated from Bauhinia bauhinioides (pep-BbKI) has potent anti-inflammatory and antioxidant effects. Purpose: To investigate the effects of pep-BbKI treatment in an ACO model and compare them with those of corticosteroids. (2) BALB/c mice were divided into groups: SAL (saline), OVA (ovalbumin), ELA (elastase), ACO (ovalbumin + elastase), ACO-pep-BbKI (treated with inhibitor), ACO-DX (dexamethasone treatment), ACO-DX-pep-BbKI (both treatments), and SAL-pep-BbKI (saline group treated with inhibitor). We evaluated: hyperresponsiveness to methacholine, bronchoalveolar lavage fluid (BALF), exhaled nitric oxide (eNO), IL-1 & beta;, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-& gamma;, TNF-& alpha;, MMP-9, MMP-12, TGF-& beta;, collagen fibers, iNOS, eNO, linear mean intercept (Lm), and NF-& kappa;B in airways (AW) and alveolar septa (AS). (3) ACO-pep-BbKI reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, neutrophils, IL-5, IL-10, IL-17, IFN-& gamma;, TNF-& alpha;, MMP-12 (AW), collagen fibers, iNOS (AW), and eNO (p > 0.05). ACO-DX reversed ACO alterations and was similar to SAL in all mechanical parameters, Lm, total cells and differentials, IL-1 & beta;(AS), IL-5 (AS), IL-6 (AS), IL-10 (AS), IL-13 (AS), IFN-& gamma;, MMP-12 (AS), TGF-& beta; (AS), collagen fibers (AW), iNOS, and eNO (p > 0.05). SAL was similar to SAL-pep-BbKI for all comparisons (p > 0.05). (4) Pep-BbKI was similar to dexamethasone in reducing the majority of alterations of this ACO model.
conferenceObject Essential oils reduced lung inflammation in a model of acute lung injury(2014) GUERREIRO, Marina; MERNAK, Marcia; SANTANA, Fernanda; PINHEIRO, Nathalia; SARAIVA-RAMANHOLO, Beatriz; CAPELLO, Tabata; TIBERIO, Iolanda; MARTINS, Milton; LAGO, Joao; PRADO, CarlaconferenceObject Preventive Effects of Inhibition of Thymic Stromal Lymphopoietin on Bronchial Hyperresponsiveness and Inflammatory Cellular Pattern in an Experimental Model of Chronic Allergic Pulmonary Inflammation Exacerbated by Lipopolysaccharide(2023) GRENCHESKI, E. A.; LOPES, B. Q. S.; CRUZ, E. L.; RIGHETTI, R. F.; SANTOS, T. M.; CAMARGO, L. D.; ALMEIDA, F.; SARAIVA-ROMANHOLO, B. M.; PRADO, C. M.; BEZERRA, S. K. M.; PIGATI, P. A.; LOPES, F. D.; LEICK, E. A.; TIBERIO, I. F. L. C.conferenceObject Particulate Matter Induces Lung Inflammation in Healthy Mice and Exacerbates Experimental Asthma Model Animals(2023) TIBERIO, I. C.; CAMPOS, E. C.; GALLI, T. T.; SANTOS, T. M.; FUKUZAKI, S.; CAMARGO, L. D.; HAMAGUCHI, S. S. S.; BEZERRA, S. K. M.; SILVA, F. J. A. da; REZENDE, B. G. de; LOPES, F. D. T. Q.; OLIVO, C. R.; SARAIVA-ROMANHOLO, B. M.; PRADO, C. M.; LEICK, E. A.; BOUROTTE, C. L. M.; BENSENOR, I. J. M.; RIGHETTI, R. F.; LOTUFO, A.- Pulmonary function evaluation on Asthma-COPD overlap from ELSA-Brasil cohort study.(2023) ALMEIDA, Francine Maria; OLIVEIRA, Roselaine Maria; QUEIROZ, Vitoria Caroline; OLIVO, Clarice Rosa; SARAIVA-ROMANHOLO, Beatriz Mangueira; LOTUFO, Paulo A.; BENSENOR, Isabela; SANTOS, Itamar Souza; TIBERIO, Iolanda Fatima Lopes Calvo