HERALDO POSSOLO DE SOUZA

(Fonte: Lattes)
Índice h a partir de 2011
19
Lattes
ResearcherID
ORCID
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/02 - Laboratório de Anatomia Médico-Cirúrgica, Hospital das Clínicas, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina

Filtros

Limpar filtros

Configurações

search.filters.applied.f.dateIssued: 2017 ×

Resultados de Busca

Agora exibindo 1 - 5 de 5
  • article 3 Citação(ões) na Scopus
    Effects of diazoxide in experimental acute necrotizing pancreatitis
    (2017) ANDRADE, Roberta de Oliveira; KUNITAKE, Tiago; KOIKE, Marcia Kiyomi; MACHADO, Marcel C. C.; SOUZA, Heraldo Possolo
    OBJECTIVE: We aimed to assess the effects of diazoxide on the mortality, pancreatic injury, and inflammatory response in an experimental model of acute pancreatitis. METHODS: Male Wistar rats (200-400 g) were divided randomly into two groups. Fifteen minutes before surgery, animals received physiological (0.9%) saline (3 mL/kg) (control group) or 45 mg/kg diazoxide (treatment group) via the intravenous route. Acute pancreatitis was induced by injection of 2.5% sodium taurocholate via the biliopancreatic duct. Mortality (n= 38) was observed for 72 h and analyzed by the Mantel-Cox Log-rank test. To study pancreatic lesions and systemic inflammation, rats (10 from each group) were killed 3 h after acute pancreatitis induction; ascites volume was measured and blood as well as pancreases were collected. Pancreatic injury was assessed according to Schmidt's scale. Cytokine expression in plasma was evaluated by the multiplex method. RESULTS: Mortality at 72 h was 33% in the control group and 60% in the treatment group (p = 0.07). Ascites volumes and plasma levels of cytokines between groups were similar. No difference was observed in edema or infiltration of inflammatory cells in pancreatic tissues from either group. However, necrosis of acinar cells was lower in the treatment group compared to the control group (3.5 vs. 3.75, p = 0.015). CONCLUSIONS: Treatment with diazoxide can reduce necrosis of acinar cells in an experimental model of acute pancreatitis, but does not affect the inflammatory response or mortality after 72 h.
  • article 2 Citação(ões) na Scopus
    Reduction of venous pressure during the resection of liver metastases compromises enteric blood flow: IGFBP-1 as a novel biomarker of intestinal barrier injury
    (2017) BARBEIRO, Hermes Vieira; MACHADO, Marcel Autran Cesar; SOUZA, Heraldo Possolo de; SILVA, Fabiano Pinheiro da; MACHADO, Marcel Cerqueira Cesar
    OBJECTIVES: Disruption of the intestinal barrier and bacterial translocation commonly occur when intestinal blood flow is compromised. The aim of this study was to determine whether liver resection induces intestinal damage. METHODS: We investigated intestinal fatty-acid binding protein and insulin-like growth factor binding protein levels in the plasma of patients who underwent liver resection. RESULTS: We show that liver resection is associated with significant intestinal barrier injury, even if the Pringle maneuver is not performed. CONCLUSION: We propose the use of insulin-like growth factor binding protein-1 as a novel biomarker of intestinal damage in such situations.
  • conferenceObject
    LIVER RESECTION INDUCES ACUTE INTESTINAL BARRIER DYSFUNCTION IGFBP-1 AS A NOVEL BIOMARKER OF INTESTINAL INJURY
    (2017) MACHADO, Marcel C. C.; BARBEIRO, Hermes; SOUZA, Heraldo P.; PINHEIRO, Fabiano; MACHADO, Marcel Autran
  • conferenceObject
    BLOOD PHOSPHOLIPIDS PROFILE OF PATIENTS SUBMITTED ROUX-EN-Y GASTRIC BYPASS SURGERY Basic science and research in bariatric surgery
    (2017) BARBOSA, C. Fernandez; FIAMONCINI, J.; ARNONI JUNIOR, J. R.; ARAUJO JUNIOR, J. C.; SZEGO, T.; TAGLIERI, C.; SOUZA, H. Possolo De; DANIEL, H.; LIMA, T. Martins De
  • article 9 Citação(ões) na Scopus
    Extracellular matrix remodeling derangement in ex-obese patients
    (2017) PRIST, Iryna Hirata; SALLES, Alessandra Grassi; LIMA, Thais Martins de; MODOLIN, Miguel Luiz Antonio; GEMPERLI, Rolf; SOUZA, Heraldo Possolo
    A known consequence of the large weight loss after bariatric surgery is the appearance of large skinfolds, particularly in the abdomen region of the patients. The balance between the synthesis of extracellular matrix (ECM) components and their proteolysis, mainly by fibrinolytic systems and matrix metalloproteases (MMPs), may be disturbed in these patients. The causes underlying the deregulation of ECM remodeling that occurs in these patients are not, however, clear. We investigated molecular mechanisms responsible for this dysfunction of ECM remodeling process, comparing it to normal skin. Collagen types, MMP2 and MMP9 expression and activity, interleukins 1 beta (IL1 beta) and 6 (IL6), and transcription coactivator PGC-1 beta expression were analyzed in 16 patients. Ex-obese patients presented increased expression of collagen types III and IV mRNA, increased expression of MMP2, decreased expression and activity of MMP9, and increased expression of PGC-1 beta in the skin. Inflammation markers IL1 beta and IL6 mRNA were not different. We have demonstrated that obese patients with extensive weight loss after bariatric surgery have increased expression of PGC-1 beta in the skin, which can result in a decreased expression and activity of MMP9 and increased collagen types III and IV deposition. These molecular changes may contribute for the formation of saggy skinfolds observed in these patients and impair wound healing.