PAOLA LARA FARIA
Projetos de Pesquisa
Unidades Organizacionais
LIM/07 - Laboratório de Gastroenterologia Clínica e Experimental, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
conferenceObject COMBINATION OF IFNL4 AND IFNL3 HAPLOTYPES IMPROVES PREDICTION OF RESPONSE TO INTERFERON THERAPIES IN PATIENTS FROM AN ADMIXED POPULATION(2014) NASTRI, A. C.; MALTA, F.; FARIA, P. L.; OLIVEIRA, K.; CARRILHO, F.; PINHO, J. R.- Increased hepatic expression of miRNA-122 in patients infected with HCV genotype 3(2016) OLIVEIRA, Ketti G.; MALTA, Fernanda M.; NASTRI, Ana C. S. S.; WIDMAN, Azzo; FARIA, Paola L.; SANTANA, Rubia A. F.; ALVES, Venancio A. F.; CARRILHO, Flair J.; PINHO, Joao R. R.Hepatitis C virus (HCV) infection affects approximately 3 % of the world population. HCV targets hepatic tissue, and most infected patients develop a chronic infection. Currently, studies have demonstrated an association between HCV-RNA replication and miR-122, the most abundant microRNA in the liver. Our aim was to evaluate liver and serum expression of miR-122 in patients infected with HCV genotypes 1 and 3, and to identify possible associations between miR-122 expression and lipid profiles, HCV viral load, apolipoproteins and liver enzymes. MicroRNAs were isolated from blood and liver tissue, and miR-122 expression was quantified by real-time PCR. HCV viral load was quantified by real-time PCR and HCV genotype, and serum biomarkers were obtained from medical report. The levels of miR-122 were higher in liver than those in blood from individuals infected with HCV genotypes 1 and 3 (p < 0.0001). The tissue levels of miR-122 were higher in subjects infected with HCV genotype 3 (6.22-fold, p < 0.001). A positive correlation was observed between the blood and hepatic levels of miR-122 in patients infected with HCV genotype 1 (r = 0.302, p = 0.026); in these patients, an inverse correlation was observed between serum apolipoprotein A-II (ApoA-II) levels and the blood (r = -0.330; p = 0.014) and hepatic (r = -0.311; p = 0.020) levels of miR-122. In patients infected with HCV genotype 3, there was a positive correlation between the hepatic miR-122 and the high-density lipoprotein-HDL (r = 0.412, p = 0.036) and insulin (r = 0.478, p = 0.044). Lipid metabolism proteins and miR-122 expression levels have different relations in HCV-3- and HCV-1-infected patients.
conferenceObject HEPATIC EXPRESSION OF miRNA AND miR-33a TARGET GENES IN CHRONIC HCV INFECTION(2014) OLIVEIRA, K.; MALTA, F.; NASTRI, A. C.; FARIA, P.; WIDMAN, A.; CARRILHO, F.; PINHO, J. R.conferenceObject Single-nucleotide polymorphisms (SNPs) in IL18BP and IFN-gamma genes as biomarker candidates for spontaneous HCV clearance prediction(2013) FARIA, Paola; MALTA, Fernanda; NASTRI, Ana Catharina S.; OLIVEIRA, Ketti G.; CARRILHO, Flair J.; PINHO, Joao R.